FastQCFastQC Report
Sun 12 May 2024
EGAF00008110163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008110163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444486866
Total Bases40 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG145278773.2684603553617713Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT99640732.2417024578629507Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC49605491.1160170028511034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGC44923761.0106881313338965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCT40741600.9165985120469228Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC26876930.6046732098491298Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTGTCCCGCGGGGCC24062810.5413615528518225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGC21758210.4895130017182555Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21198400.4769184788465718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA20729960.46637958476820324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGC17801800.40050227265882815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16965630.3816902432388182No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT14731160.3314194665090509Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA14219100.3198992160996721Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAGG13896710.3126461333955366No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGCCC13775680.30992321829369873Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTA13588610.3057145450052511Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGCCG12131580.2729344988114902Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGCCGGGCGCGACCCGC11897130.26765987726620477Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCCGGCCGGGCGCGACCC10570920.23782300015137003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAACAAAGCATCGCGAAG9958060.2240349661985288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC9550340.21486214173086501Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCG9004210.20257538948293694Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA8831430.19868821050833932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAGCTAG8806800.1981340883984635Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAA8724260.1962771156437275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAGTGTCCCGCGGGGCCC8420150.18943529368537068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA8392480.18881277810354918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGCC7333160.1649803528727888Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG7274490.16366040385994216Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTGTCCCGCGGGGCCCG6689720.15050433458702017Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGCC6595900.14839358605480144Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6262340.14088920233697072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG6069480.1365502664818897Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTACAGTGAAACTGCGAAT5965900.1342199389081611Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGA5863610.13191863356430425Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC5344380.12023707355168511Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA5178510.11650535473864823No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG4971950.1118581983027593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGCCTGGATACCGCAGCT4958050.11154547815142865Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGCTA4928330.11087684197174906Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCCCGCCACGCAGTTTTA4826240.10858003619841491Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG4734920.10652553229773049Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGCCCG4628880.10413986000657215Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGCCCGAA4560600.1026037066301077No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)