FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008115852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47748327
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33296586.973350081982978No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19645304.114343105675723No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16975543.555211473692052No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16841033.5270408531800497No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15224653.188520092023329No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12973232.7170020009287446No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4503950.9432686510670835No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4372420.9157221361912847No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2850420.5969675125999703No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2693850.5641768349286876No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2680130.5613034358250919No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2645800.5541136551234559No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2598360.5441782284853666No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2543700.5327307069837233No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2456840.5145394937083345No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2317190.48529239568959137No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC2231210.4672854820651622No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2073830.4343251649424283No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2008480.42063882154447No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1838120.38496008457008346No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1775530.37185177189558916No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1702390.35653395772379626No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1581620.33124092494382057No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1539410.322400824640411No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1532150.3208803525199951No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1523200.31900594129716836No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1502380.31464557910060387No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1398770.2929463895143384No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1398480.29288565440209036No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1386230.29032011948816555No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1335480.2796914748447626No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1266300.2652030091022875No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG1178320.24677723263476856No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1165930.24418237732182743No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1156580.24222419353038274No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1145910.23998956026249885No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA1123030.23519776933755188No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1093870.22909074908530302No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1000110.20945445900125464No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC983960.20607214154330475No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA923050.19331567365700583No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC890700.18654056717002881No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA877670.18381167574729895No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA864260.18100320038438206No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT839700.1758595646712397No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG837030.17530038277571483No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830050.17383855145333155No Hit
CAGTAGTCACGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA813870.17044995105273533No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA801940.16795143419370484No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG797100.1669377861134276No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG789530.16535239025233284No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGC784430.1642842900024539No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG774400.1621836928443587No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT759030.15896473189521385No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC758450.1588432616707178No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG752990.15769976610908273No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA731070.15310902934881884No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG722340.151280693038732No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA703750.1473873629122126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG694700.1454920085472314No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG686110.14369299263616084No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC674950.14135573797171994No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT665850.13944991203566148No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA646620.13542254579935334No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA635660.1331271774192214No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT627160.1313470103360899No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG620670.1299878004102636No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG609590.12766730025954626No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG599690.12559392918625192No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG593050.12420330454719387No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA582990.12209642444645234No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA577080.12085868474512207No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG566140.11856750499342102No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA565280.11838739397089242No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG541490.11340502045233962No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA539930.11307830743472959No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCA535630.1121777523220866No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA512080.10724564234470454No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA511220.10706553132217594No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC495550.10378374094656762No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG494080.10347587675689662No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT479810.10048729037145114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)