FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008115858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59925056
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59081259.859189785321185No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26481064.419029662650628No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23667453.949508199041149No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12311042.0544060901670247No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10057621.678366391514094No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT8812371.4705651672649251No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7624291.272304192757033No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC7125741.1891086092602068No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG5663640.9451205185356856No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4452300.7429780290902023No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4433550.7398491208752479No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3971890.6628095599944037No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC3577550.5970040311685316No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3502770.5845251108317696No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2970880.49576591134099235No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2880870.48074548315816346No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA2736520.45665706178063475No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2568000.428535269120149No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2507240.41839593775264894No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2341240.390694670356253No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG2337920.39014064500832507No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC2318970.3869783617724112No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1950040.3254131293594453No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA1932890.322551221312167No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1730010.28869560005083683No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1674910.27950078177649096No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT1621160.2705312448936218No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1568330.2617152331071664No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1566080.2613397641213719No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG1565020.26116287651028647No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1516750.25310781520170794No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGC1434760.23942572535935552No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT1434470.23937733157896424No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1427460.2382075370943333No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA1343730.22423508456963312No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC1340200.2236460154496977No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1337600.22321214017722402No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA1317040.2197811880225861No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG1306630.21804401818164343No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG1295850.21624510455192564No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1229760.20521632887585453No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA1228070.20493430994874665No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1167880.19489009739098118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1146100.19125555760849017No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT1143280.19078496981296103No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1143250.1907799635598171No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1142460.19064813222702703No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA1118080.18657971717206237No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1114070.18591054800182416No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC1077690.1798396316892887No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1001700.16715879247572168No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG970780.16199901423538096No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG953570.15912709368181482No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG949430.1584362307479529No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT947220.1580674367663503No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA889430.14842372446009897No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA873030.14568697274141887No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCA865110.14436532191142215No Hit
CAGTAGTCACGGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC864250.14422180932129627No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT853200.14237783941328316No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGG849910.14182882031849917No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTG818090.13651885448384063No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT809240.13504200980638215No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA786670.1312756386911011No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA768020.1281634179866265No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCG725320.12103785101177043No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG720020.12015341295634333No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCA713910.1191338060660302No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA710530.11856976821181445No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT707220.1180174116149345No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG701960.1171396485636993No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGC686470.114554753190385No Hit
CAGTAGTCACGGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC685770.11443794061702672No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC680230.11351345253644818No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA679430.11337995245261014No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAT661890.11045296311446083No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG637380.10636285429587249No Hit
CAGTAGTCACGGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT632790.10559689756485166No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATC627260.10467407823532113No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGG621160.10365614009605598No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG618670.10324062108511005No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA609410.10169535761468458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)