FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008115881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6084185
Sequences flagged as poor quality0
Sequence length109
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC766611.2600044212988264No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC496370.8158364678260113No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT419870.6901006461835069No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC278760.4581714724322157No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG138720.2280009565784078No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT127790.2100363483358905No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT101920.16751627374907238No Hit
TGGCATTGTATTAGCAACCTCATCACTAGACATCGTACTACACGACACGT88460.1453933435620383No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA81940.13467703562597128No Hit
AGGGGTCCAGCCTCGTTGGAAGAGGAACAGCACTGGGGAGTCTTTGTGGA75600.12425657668200425No Hit
TTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTTAAAAAATATAG74270.12207058135148752No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC72190.11865188188722071No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA71340.11725481720230402No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT67640.11117347680913714No Hit
CGTAGACCAGAACCAATTTAGAAGAATACTTGAAGCTAGAAGGGGAAGTT61950.10182136144775349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)