FastQCFastQC Report
Wed 8 May 2024
EGAF00008115882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6084185
Total Bases663.1 Mbp
Sequences flagged as poor quality0
Sequence length109
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC532770.8756637084506799No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC372140.6116513551116542No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT296460.48726328998871665No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC244320.4015656986104137No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT85330.14024885831052147No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG82460.13553171049203797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80440.1322116273584712No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC73710.12115016226495413No Hit
AACACCCTCATCTTCTCAAGCTTTACCTTCTAACTTCTGCACCACCAGAA71600.11768215463533735No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT69150.11365532113175388No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT60960.10019419199120343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)