FastQCFastQC Report
Wed 8 May 2024
EGAF00008115885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6914994
Total Bases753.7 Mbp
Sequences flagged as poor quality0
Sequence length109
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC836921.210297507127266No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC368240.5325239616983037No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC367020.5307596796179432No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC264970.38318182199435025No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT228230.33005090098415124No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG165340.23910360587442303No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG149590.21632701344354022No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG141190.20417949748040273No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA140340.20295028455556147No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG134370.1943168714246173No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT127550.18445424536883184No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA108220.15650049732508806No Hit
GGGCTCATCTCCCCGCGTCCGGCTTGGATATCTTCGGGGACCTGAAGAAG108170.15642819068245034No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG107450.15538697502846713No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG106040.15334792770608335No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC105590.1526971679223438No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA98320.14218378208281887No Hit
CGGCCCCCCATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT94830.1371367784267058No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC89640.12963134892091013No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT86370.12490249449240304No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT71410.10326834701519624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)