FastQCFastQC Report
Wed 8 May 2024
EGAF00008115903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273657
Total Bases683.8 Mbp
Sequences flagged as poor quality0
Sequence length109
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACTCGCCCAGCCCAGCCTCCGGGCCACCCAGCCTGTGGAGGTTGGT392150.625074019826076No Hit
AGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTC299300.4770742168403532No Hit
AGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCC263230.41957983995618503No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC262220.41796993364476254No Hit
AGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCC242250.3861384197446561No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA212930.3394033177140542No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG203210.3239099619249188No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA167960.26772263768962823No Hit
GAGATACTCGCCCAGCCCAGCCTCCGGGCCACCCAGCCTGTGGAGGTTGG129080.2057492145330865No Hit
CGGCCCCCCGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACA112030.1785720832363006No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG107990.17213245799061058No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC107090.17069788801013508No Hit
CGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGG106330.16948647335995576No Hit
TGGCGGGTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGAC101610.16196295079568424No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG81270.12954166923693788No Hit
TGGCTGGCAGGAACTGGGGTGGCTATCAATATTGGATGGCGAGGACAGCG70680.11266156246667614No Hit
TGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATG67870.10818251619430262No Hit
AAAATGGAGAGCCTCTCAAGAAAAAGAGCTATGCAGTCAGCAATGACTTA63660.1014719166189672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)