FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008115919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6082433
Sequences flagged as poor quality0
Sequence length109
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACTCGCCCAGCCCAGCCTCCGGGCCACCCAGCCTGTGGAGGTTGGT128060.21054074907195852No Hit
CGGCCCCCCGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACA98710.16228703217939267No Hit
CGGCCCCCCGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCA80730.13272649283600824No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT74060.12176048630539785TruSeq Adapter, Index 2 (97% over 37bp)
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG61370.1008971245552561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)