FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008115933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008115933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47699668
Sequences flagged as poor quality0
Sequence length100
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC9058921.8991578725453602No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT7574081.587868494179037No Hit
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG6166991.2928790196191722No Hit
GGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGG5955911.2486271392916195No Hit
GCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT5689981.192876227146906No Hit
GCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTA4572740.9586523746873878No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG3825540.8020055820933597No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC3351210.7025646383953867No Hit
CCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTAT3013050.6316710632032072No Hit
CTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTC2928120.6138659078297988No Hit
GCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGC2778410.5824799451434337No Hit
GGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGA2725940.5714798685810559No Hit
GCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTG2544730.5334900863460936No Hit
CGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGAC2310710.4844289482266418No Hit
CGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTC2249180.47152948737504846No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC2227890.46706614394045676No Hit
GCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGC2201820.4616006970949987No Hit
GGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCC2122610.44499471149358943No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC1985240.4161957689097543No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA1951450.4091118621622272No Hit
CGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATG1898830.39808033884009425No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT1885170.3952165872517184No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG1845470.3868936781698355No Hit
CGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCG1835600.3848244813779417No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC1752560.36741555517744906No Hit
CGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGG1725070.36165241233964146No Hit
GCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGA1683730.3529856853511014No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1639140.3436376119011981No Hit
CGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGG1613050.33816797215443933No Hit
CTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCG1583590.3319918285385131No Hit
CGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGCC1504640.31544035065401294No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG1495330.3134885550985386No Hit
GCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCA1480780.3104382194022818No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1450910.3041761213096913No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA1442840.3024842856348602No Hit
CTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGG1425350.29881759344740094No Hit
CGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCC1359630.28503971977331166No Hit
CGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCC1339270.2807713462492024No Hit
CGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACACCTC1332220.2792933485407068No Hit
GTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGG1331810.27920739406404255No Hit
CGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCC1327050.2782094835544767No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG1326400.2780732142622041No Hit
GCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGG1284100.2692052280112306No Hit
GCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGG1187640.24898286503797049No Hit
GCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACG1174250.24617571761715407No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT1170410.2453706805674203No Hit
GCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGG1156560.24246709641668784No Hit
CGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTC1146970.24045660024300378No Hit
GCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGA1138750.23873331780841744No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT1106680.23201000057275029No Hit
CGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCG1081880.22681080295988643No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA1070370.22439778826133547No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAA1035270.2170392464786128No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC1033900.2167520327395151No Hit
CGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGG1025950.21508535447248814No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC1021790.21421323100194326No Hit
GCCGCCGCCGCCCTCCGACGCACACCACACGCGCGCGCGCGCGCGCCGCC1005640.21082746320163068No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1005120.21071844776781257No Hit
GCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCG993330.20824673245105185No Hit
CGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG981400.20574566682518627No Hit
GCGGCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCG932200.19543112962547246No Hit
GGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGC928770.19471204705240297No Hit
CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC922040.19330113576471855No Hit
GCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTA918420.19254222062929244No Hit
GCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCA913050.19141642663005537No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT905110.1897518448136788No Hit
CGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGC881890.18488388640356992No Hit
CACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAG864140.18116268649920164No Hit
GCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCT861800.18067211704702013No Hit
CCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGG860360.18037022815336995No Hit
GGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCG839380.17597187468893916No Hit
GCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTC834320.17491107065986286No Hit
CGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTCCTACTCG828180.173623849960549No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC826100.17318778822527653No Hit
CGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGC816880.17125486072565535No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAG815780.17102425115411704No Hit
CGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGC814900.1708397634968864No Hit
CACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTG800970.16791940774095115No Hit
CGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGT796880.16706195942495866No Hit
CGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATA793240.1662988513882319No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG785340.16464265537445671No Hit
GCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGA782460.16403887758715635No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACG775130.16250217926045105No Hit
GGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCT758940.15910802565753707No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC746740.1565503558641121No Hit
GCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTC728620.15275158728568092No Hit
CGGCGCTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTT705310.14786476081971892No Hit
CGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACG696300.14597585878375505No Hit
GGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCAT695160.14573686340961536No Hit
CCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTG692390.14515614657946885No Hit
GCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGC682930.14317290426423931No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC663920.13918755157792712No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA648520.13595901757639067No Hit
GGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTA643960.13500303607983183No Hit
GCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCG629930.1320617158173931No Hit
CGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGC625930.13122313555725376No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT623700.1307556270622261No Hit
CCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGA622660.13053759619458988No Hit
CGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAA620710.13012878831777194No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG609640.12780801744783632No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC608350.1275375753139414No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA605760.12699459459550116No Hit
GCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTC601530.12610779597040384No Hit
AATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGG600250.12583945028715923No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA590230.12373880673551019No Hit
CGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACT582380.12209309297498674No Hit
TGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG580460.12169057445011987No Hit
TGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACT579050.12139497490842074No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT576380.12083522258477773No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT575420.12063396332234429No Hit
GCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGC573790.12029224186633752No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT569010.119290138455471No Hit
CCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCC568680.11922095558400951No Hit
CGCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCC565760.11860879199410779No Hit
GCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCC563800.11819788766663952No Hit
GCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCG562960.11802178581201027No Hit
CTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTC560960.11760249568194059No Hit
GCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTG559560.11730899259089184No Hit
GCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC550010.11530688221980917No Hit
GCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAG548760.11504482588851563No Hit
CGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCT543270.1138938744814744No Hit
CCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTA538620.11291902492906239No Hit
GTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTC536160.11240329806907672No Hit
GGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGAC535750.11231734359241241No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC534300.11201335824811193No Hit
ACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTC532770.11169260129860863No Hit
CCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTCCTACTC519580.10892738289079915No Hit
CGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACG517280.10844519924121904No Hit
CCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTCCTACT511830.10730263363677918No Hit
GCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCC508290.10656049010655587No Hit
GCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGC507100.10631101247916441No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC506990.1062879515220106No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC499970.10481624316546606No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT494060.10357724083111018No Hit
CAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCG493800.10352273311420113No Hit
CCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATAT490550.10284138665283792No Hit
GCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATG487100.10211811117846774No Hit
GGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGC486090.10190636966278258No Hit
GGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTA481090.10085814433760838No Hit
ATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGG479140.10044933646079046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)