FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008116193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008116193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893015
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATCAATCTCGTAT965560.7489016339467534TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATCAATCGCGTAT350250.27165872373529387TruSeq Adapter, Index 12 (97% over 37bp)
GGAGGAAGAGACTTTATTTGGCCCCAGCCCCTAGCCCCACAGCCAAGACA146810.1138678579060057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)