FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008116227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008116227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14251614
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGAGAGATCTCGTAT1431401.004377469106306TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGAGAGATCGCGTAT325340.2282829158858779TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGCGAGATCTCGTAT311650.21867698633993315TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGCGAGATCGCGTAT188760.13244815639828583TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)