FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008116293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008116293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12793833
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAACTGGATCTCGTAT787810.6157732401227998TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCGGAACTCCAGTCACTCAACTGGATCTCGTAT379260.29643969872046944TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAACTGGATCGCGTAT174560.13644073672057466TruSeq Adapter, Index 3 (97% over 36bp)
GGAGGAAGAGACTTTATTTGGCCCCAGCCCCTAGCCCCACAGCCAAGACA155600.12162109666430693No Hit
GATCGGAAGAGCACACGTCGGAACTCCAGTCACTCAACTGGATCGCGTAT136540.10672329394951457TruSeq Adapter, Index 3 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)