FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008116391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008116391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12230257
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCTTGTATCTCGTAT544300.44504379589079773TruSeq Adapter, Index 13 (97% over 37bp)
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC426070.3483737095630942No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCTTGTATCGCGTAT213510.17457523582701492TruSeq Adapter, Index 13 (97% over 37bp)
CTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGG141120.11538596449772069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)