FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89321
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19862.223441295999821No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19582.1920936845758554No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15051.6849341140381322No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13481.5091635785537556No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13121.468859506722943No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12361.3837731328578946No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10561.1822527737038322No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9781.094927284737072No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8740.9784932994480581No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8120.9090807312949922No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7460.8351899329385027No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5640.6314304586827286No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5290.592245944402772No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4760.5329093942074092No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4620.5172355884954266No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4260.4769315166646142No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3850.4310296570795222No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3800.4254318693252427No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3730.41759496646925137No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3440.3851277974944302No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2870.3213130170956438No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2820.3157152293413643No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2790.3123565566887966No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2710.30340009628194936No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2660.29780230852766987No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2650.29668275097681396No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2260.2530200064934338No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2230.24966133384086608No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2070.23174841302717164No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2060.23062885547631579No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1970.22055283751861265No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1960.21943327996775674No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1930.21607460731518902No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1910.21383549221347725No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1860.2082377044591977No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1860.2082377044591977No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1840.20599858935748594No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1810.2026399167049182No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1800.2015203591540623No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1770.19816168650149463No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1700.1903247836455033No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1680.1880856685437915No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1590.17800965058608836No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1550.17353142038266478No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1490.16681407507752935No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1430.16009672977239395No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1420.15897717222153804No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1410.15785761467068216No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1390.15561849956897034No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1390.15561849956897034No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1390.15561849956897034No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1350.15114026936554673No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1330.14890115426383493No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1330.14890115426383493No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1320.14778159671297902No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1300.14554248161126723No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1300.14554248161126723No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1290.14442292406041132No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1280.1433033665095554No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1280.1433033665095554No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1270.14218380895869953No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1250.1399446938569877No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1230.13770557875527592No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1220.13658602120442No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1210.1354664636535641No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1190.1332273485518523No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1190.1332273485518523No Hit
GACTACTGGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC1190.1332273485518523No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1180.1321077910009964No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1180.1321077910009964No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1170.13098823345014052No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1160.1298686758992846No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1150.1287491183484287No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1140.1276295607975728No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1120.125390445695861No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1120.125390445695861No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1120.125390445695861No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1110.12427088814500509No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1110.12427088814500509No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCAC1100.1231513305941492No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1090.1220317730432933No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1080.12091221549243739No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1080.12091221549243739No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1070.11979265794158148No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1050.11755354283986968No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1050.11755354283986968No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1040.11643398528901377No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCAC1040.11643398528901377No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1030.11531442773815789No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1010.11307531263644607No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1000.11195575508559016No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC980.10971663998387837No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA980.10971663998387837No Hit
GACTACTCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC970.10859708243302246No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA960.10747752488216658No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG950.10635796733131067No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT940.10523840978045476No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC940.10523840978045476No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA930.10411885222959885No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA900.10076017957703115No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT900.10076017957703115No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG900.10076017957703115No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG900.10076017957703115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)