FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150375
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG76565.091271820448878No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG67064.4595178719867No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG55873.7153782211138817No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG52453.4879467996674975No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG44952.9891936824605154No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG42382.8182876142975894No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41122.7344970906068164No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG36142.40332502078138No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG32272.145968412302577No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18941.2595178719867No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16581.1025768911055693No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14260.9482959268495428No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13380.8897755610972568No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11380.7567747298420615No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10790.717539484621779No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10580.7035743973399834No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8990.5978387364921031No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7970.5300083125519535No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7960.5293433083956774No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6790.4515378221113882No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6460.42959268495428093No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6230.41429758935993355No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5740.3817123857024107No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5570.37040731504571905No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5540.3684123025768911No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5490.36508728179551125No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5420.3604322527015794No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5000.3325020781379884No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4870.32385702410640066No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4720.313881961762261No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4610.3065669160432253No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4400.29260182876142976No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4380.29127182044887784No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4350.28927680798004984No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4230.28129675810473814No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4230.28129675810473814No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4200.2793017456359102No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4190.27863674147963424No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4160.2766417290108063No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4120.27398171238570246No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4110.27331670822942644No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3960.2633416458852868No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3860.256691604322527No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3810.25336658354114716No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3770.2507065669160432No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3750.24937655860349126No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3720.24738154613466334No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3580.23807148794679966No Hit
GACTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3560.23674147963424771No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3550.23607647547797175No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3490.23208645054031588No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3440.22876142975893599No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3430.22809642560266No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3220.2141313383208645No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3180.21147132169576058No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3170.21080631753948462No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3130.20814630091438072No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3090.2054862842892768No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3080.20482128013300083No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3020.200831255195345No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2960.19684123025768913No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2950.19617622610141314No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2860.19019118869492935No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2850.18952618453865336No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2750.1828761429758936No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2740.18221113881961765No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2730.18154613466334163No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2680.17822111388196177No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2600.17290108063175394No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2590.17223607647547798No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2560.17024106400665004No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2550.16957605985037405No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2540.1689110556940981No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2520.16758104738154614No Hit
GACTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2510.16691604322527015No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2500.1662510390689942No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2480.1649210307564422No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2450.1629260182876143No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2440.1622610141313383No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2380.15827098919368246No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2370.1576059850374065No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2320.1542809642560266No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2310.15361596009975062No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2300.15295095594347463No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2270.15095594347464672No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2210.14696591853699087No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2200.14630091438071488No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2200.14630091438071488No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2180.14497090606816293No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2140.142310889443059No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2080.13832086450540315No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2060.13699085619285123No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2050.1363258520365752No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2050.1363258520365752No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2040.13566084788029925No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.1310058187863674No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1960.13034081463009142No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1940.1290108063175395No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1910.12701579384871156No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1880.12502078137988362No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1870.12435577722360765No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1800.11970074812967581No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1780.11837073981712386No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1760.1170407315045719No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.11571072319201996No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1730.11504571903574398No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1720.11438071487946799No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1710.11371571072319202No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.11172069825436409No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1660.11039068994181213No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1660.11039068994181213No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1660.11039068994181213No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1660.11039068994181213No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1630.1083956774729842No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1610.10706566916043225No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.10440565253532835No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1560.10374064837905238No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1560.10374064837905238No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1530.10174563591022444No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.10174563591022444No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1530.10174563591022444No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1510.1004156275976725No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1510.1004156275976725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)