FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008118596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59540
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA8751.4696002687269063No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7831.3150822976150487No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA6361.0681894524689284No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5971.002687269062815No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA5540.9304669129996642No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4970.8347329526368827No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4820.809539805172993No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4060.6818945246892846No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3800.6382264024185421No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2840.47699025864964734No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2530.4249244205576083No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2340.39301310043668125No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2260.37957675512260663No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2180.3661404098085321No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2010.3375881760161236No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1950.32751091703056767No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1910.3207927443735304No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1820.3056768558951965No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1770.2972791400738999No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1690.2838427947598253No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1680.28216325159556604No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1610.27040644944575076No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA1590.2670473631172321No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1530.2569701041316762No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA1480.2485723883103796No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA1440.2418542156533423No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1410.23681558616056433No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1410.23681558616056433No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA1380.2317769566677864No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1370.23009741350352703No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1360.2284178703392677No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1340.22505878401074905No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1330.22337924084648975No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA1300.21834061135371177No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1290.21666106818945247No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA1280.21498152502519313No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1260.2116224386966745No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA1240.20826335236815585No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA1210.2032247228753779No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA1200.20154517971111857No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA1200.20154517971111857No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1170.19650655021834063No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA1170.19650655021834063No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA1170.19650655021834063No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA1160.1948270070540813No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA1140.19146792072556262No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1140.19146792072556262No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1130.18978837756130332No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA1130.18978837756130332No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA1120.18810883439704398No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA1100.18474974806852534No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1100.18474974806852534No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA1100.18474974806852534No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA1080.1813906617400067No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.1797111185757474No Hit
GANTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1070.1797111185757474No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1060.17803157541148806No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA1060.17803157541148806No Hit
GNNTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1030.17299294591871012No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1010.16963385959019148No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA990.1662747732616728No Hit
GANTANCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA990.1662747732616728No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG980.1645952300974135No Hit
GANTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA980.1645952300974135No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA980.1645952300974135No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC970.16291568693315417No Hit
GANTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA970.16291568693315417No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA960.16123614376889486No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA960.16123614376889486No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA960.16123614376889486No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA940.15787705744037622No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA940.15787705744037622No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA940.15787705744037622No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA930.1561975142761169No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC920.15451797111185758No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG900.15115888478333894No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA890.1494793416190796No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA880.1477997984548203No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA870.14612025529056097No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA870.14612025529056097No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA860.14444071212630166No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA850.14276116896204233No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA850.14276116896204233No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA840.14108162579778302No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA840.14108162579778302No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA840.14108162579778302No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC830.13940208263352366No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG820.13772253946926435No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.13604299630500502No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA800.13436345314074571No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA790.13268390997648638No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA790.13268390997648638No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG790.13268390997648638No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG790.13268390997648638No Hit
GANTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA770.12932482364796774No Hit
GANTANAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA770.12932482364796774No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA760.12764528048370843No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA760.12764528048370843No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA760.12764528048370843No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA750.1259657373194491No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC750.1259657373194491No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.1242861941551898No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA740.1242861941551898No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA730.12260665099093047No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA720.12092710782667115No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA710.11924756466241183No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA710.11924756466241183No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA710.11924756466241183No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA710.11924756466241183No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG700.11756802149815251No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA700.11756802149815251No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA700.11756802149815251No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC690.1158884783338932No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTANCCNNACTTTCGAGCCNCAA680.11420893516963385No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTANCCNCANTTTCGAGCCTCAA680.11420893516963385No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA680.11420893516963385No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG670.11252939200537453No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA670.11252939200537453No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC670.11252939200537453No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA660.1108498488411152No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG660.1108498488411152No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG650.10917030567685589No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTANCCNCACTTTCGAGCCTCAA650.10917030567685589No Hit
NANTANACGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA650.10917030567685589No Hit
GANTANACGGGTNTCTNNTNNTGTTTGATACCCACANTTTCGAGCCTCAA640.10749076251259657No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTANCNNCANTTTCGAGCCTNNA640.10749076251259657No Hit
GANTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA640.10749076251259657No Hit
NANTANAAGGGTATNTANTNNTGTTTGNTANCCNNANTTTCGAGCCTCAA630.10581121934833725No Hit
GANTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA620.10413167618407793No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA620.10413167618407793No Hit
GANTANACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA620.10413167618407793No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTANCCNCACNTTCGAGCCTCAA620.10413167618407793No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA620.10413167618407793No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG620.10413167618407793No Hit
GANTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA610.1024521330198186No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA610.1024521330198186No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA610.1024521330198186No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTANCCNCANTTTCGAGCCNNNA610.1024521330198186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)