FastQCFastQC Report
Wed 8 May 2024
EGAF00008118598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102551
Total Bases30.8 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG51625.033593041511053No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG48194.699125313258769No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG37323.639164903316398No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG36053.5153240826515586No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG33893.3046971750641148No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG28462.7752045323790115No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG28402.769353784946027No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG24482.387104952657702No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22472.191104913652719No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15421.5036420902770329No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14211.3856520170451776No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11311.1028658911175901No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11101.0823882751021443No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9980.9731743230197657No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9720.9478210841434994No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9510.9273434681280535No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8800.8581096235044028No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8290.8083782703240339No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7800.7605971662879932No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7790.7596220417158293No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7300.7118409376797886No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6590.642607093056138No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6270.6114031067468869No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6090.5938508644479332No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5890.5743483730046514No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5620.5480200095562209No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5570.5431443866954003No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4750.46318417177794463No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4720.46025879806145237No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4300.4193035660305604No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4190.4085771957367553No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4160.40565182202026306No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3970.3871244551491453No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3730.36372146541720707No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3680.3588458425563866No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3630.3539702196955661No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3460.3373931019687765No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3380.3295921053914638No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3340.32569160710280737No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3140.3061891156595255No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3070.29936324365437683No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3070.29936324365437683No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2930.28571149964407955No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2850.2779105030667668No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2830.2759602539224386No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2780.2710846310616181No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2760.26913438191728994No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2690.2623085099121413No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2670.2603582607678131No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2670.2603582607678131No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2590.25255726419050034No Hit
GACTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2570.25060701504617217No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2530.2467065167575158No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2470.24085576932453123No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2420.23598014646371074No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2300.22427865159774163No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2270.22135327788124934No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2270.22135327788124934No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2250.21940302873692114No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2250.21940302873692114No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2240.21842790416475702No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2210.21550253044826476No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2170.2116020321596084No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2140.2086766584431161No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2110.20575128472662382No Hit
GACTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2090.20380103558229565No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2050.19990053729363927No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1990.19404978986065471No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1970.1920995407163265No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1960.19112441614416242No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1950.1901492915719983No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1930.18819904242767013No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1920.18722391785550604No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1920.18722391785550604No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1900.18527366871117784No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1890.18429854413901375No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.18137317042252146No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1830.1784477967060292No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1800.1755224229895369No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1780.17357217384520873No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1690.1647960526957319No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1660.1618706789792396No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1650.1608955544070755No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1650.1608955544070755No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1610.15699505611841913No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1600.15601993154625504No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1590.15504480697409095No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1580.15406968240192684No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1580.15406968240192684No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1530.14919405954110637No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.14919405954110637No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1520.14821893496894228No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1480.1443184366802859No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1460.1423681875359577No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1460.1423681875359577No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1440.14041793839162953No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1440.14041793839162953No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1430.13944281381946544No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1430.13944281381946544No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1370.13359206638648088No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1360.13261694181431677No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1350.13164181724215268No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1320.12871644352566042No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1300.12676619438133221No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1290.12579106980916813No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1290.12579106980916813No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1270.12384082066483994No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1260.12286569609267584No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1260.12286569609267584No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1250.12189057152051175No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1230.11994032237618356No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1230.11994032237618356No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1200.11701494865969128No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1190.11603982408752718No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.11408957494319899No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1160.1131144503710349No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1140.1111642012267067No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.10823882751021444No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1080.10531345379372214No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1080.10531345379372214No Hit
GANTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.10433832922155804No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1050.10238808007722988No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1050.10238808007722988No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1050.10238808007722988No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1030.10043783093290168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)