FastQCFastQC Report
Wed 8 May 2024
EGAF00008118608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60247
Total Bases18.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13792.2889106511527544No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12752.1162879479476158No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10651.7677228741680084No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9221.5303666572609425No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9111.5121084867296297No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8531.4158381330190715No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6901.1452852424187097No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6391.060633724500805No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5860.9726625392135708No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2830.4697329327601374No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2800.464753431706143No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2620.43487642538217675No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2380.3950404169502215No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2380.3950404169502215No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2070.3435855727256129No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1940.3220077348249706No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1850.3070692316629874No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1790.29711022955499855No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1780.2954503958703338No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1730.2871512274470098No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1450.24067588427639552No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1430.2373562169070659No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1430.2373562169070659No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1410.23403654953773634No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1380.22905704848374195No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1310.21743821269108837No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1260.20913904426776436No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1200.1991800421597756No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1180.195860374790446No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC1180.195860374790446No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1170.1942005411057812No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1140.1892210400517868No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1140.1892210400517868No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC1130.18756120636712204No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1080.17926203794379802No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1060.17594237057446843No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1040.17262270320513887No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1030.17096286952047404No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.16764320215114445No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC980.16266370109715006No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.16266370109715006No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA920.15270469898916128No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC910.15104486530449648No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC910.15104486530449648No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC900.1493850316198317No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC880.14606536425050212No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA870.1444055305658373No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.1444055305658373No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC830.1377661958271781No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA810.13444652845784855No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG780.12946702740385413No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC780.12946702740385413No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG780.12946702740385413No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC760.12614736003452454No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG760.12614736003452454No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA750.12448752634985974No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA730.12116785898053015No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA710.11784819161120055No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA700.11618835792653576No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCTCACGCTTTCGCGCCTCAC680.11286869055720616No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG680.11286869055720616No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC680.11286869055720616No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA660.10954902318787657No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA660.10954902318787657No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA650.10788918950321179No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC630.10456952213388218No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG630.10456952213388218No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA630.10456952213388218No Hit
GACTACCAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA630.10456952213388218No Hit
GACTACTGGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT620.10290968844921738No Hit
GACTACTGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA620.10290968844921738No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.10124985476455259No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTCCCTCCACGCGGCGTCGC610.10124985476455259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)