FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008118635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38049
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17604.625614339404452No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14323.763568030697259No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13263.4849798943467634No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12433.2668401272043943No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11162.933060001576914No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8422.212935951010539No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7301.9185786748666196No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5091.3377486924754922No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3901.0249940865725775No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3270.8594181187416225No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2990.7858287997056427No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2970.7805724197745013No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2910.764803279981077No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2800.7358931903597993No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2530.66493206128939No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2500.6570474913926778No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2250.59134274225341No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2060.5414071329075666No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2040.5361507529764251No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2000.5256379931141423No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1830.48095876369944024No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1760.46256143394044524No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1670.4389077242503088No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1530.40211306473231884No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1510.3968566848011774No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1490.391600304870036No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1490.391600304870036No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1230.3232673657651975No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1200.3153827958684854No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1150.3022418460406318No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1130.2969854661094904No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1080.2838445162816368No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1050.2759599463849247No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1030.2707035664537833No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT970.25493442666035904No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT940.24704985676364688No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT850.22339614707351046No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG820.21551157717679834No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT730.19185786748666195No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC710.18660148755552053No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT690.1813451076243791No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT690.1813451076243791No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT690.1813451076243791No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT680.17871691765880837No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT650.17083234776209624No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT650.17083234776209624No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT630.16557596783095482No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT610.1603195878998134No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT610.1603195878998134No Hit
CATACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT600.1576913979342427No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG590.15506320796867198No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT590.15506320796867198No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT580.15243501800310127No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT560.14717863807195983No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT540.1419222581408184No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.1392940681752477No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT530.1392940681752477No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT530.1392940681752477No Hit
ACTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT520.136665878209677No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT520.136665878209677No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG510.13403768824410628No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT510.13403768824410628No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT510.13403768824410628No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT500.13140949827853557No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT470.12352492838182344No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT470.12352492838182344No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT470.12352492838182344No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT460.12089673841625273No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT450.11826854845068202No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT440.11564035848511131No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAACGTCAGATGTGTACATAGCGGAT430.11301216851954059No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG430.11301216851954059No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT420.11038397855396988No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT420.11038397855396988No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT420.11038397855396988No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT420.11038397855396988No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT420.11038397855396988No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT410.10775578858839917No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT410.10775578858839917No Hit
CATACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT400.10512759862282846No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT400.10512759862282846No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT400.10512759862282846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)