FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188912
Sequences flagged as poor quality0
Sequence length301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG161938.571715931227237No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG142437.539489286016769No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG121786.446387736088761No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG113716.01920470907089No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG98845.232065723723215No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG92454.893812992292708No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG90154.772063182857627No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG74673.952634030659778No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG66843.5381553315829595No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG41572.200495468789701No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG36991.9580545439146269No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG31611.6732658592360463No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG27831.4731726941644787No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25971.3747141526213262No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25191.3334250868129076No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG23151.2254382993139663No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19211.0168755822816973No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG17510.9268865926992461No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12510.6622130939273312No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11040.5843990852883882No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9220.48805793173541123No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8780.46476666384348264No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7620.4033624121283984No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7200.38112983823155755No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6270.33190056745998137No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6190.32766579147963076No Hit
GACTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5920.3133734225459473No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5420.28690607266875584No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5250.2779071737105107No Hit
GACTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5000.26467349877191493No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4780.25302786482595074No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4190.22179639197086473No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4190.22179639197086473No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4150.2196790039806894No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3840.2032692470568307No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3720.19691708308630473No Hit
GANTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3680.1947996950961294No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3370.1783899381722707No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3360.17786059117472686No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2920.15456932328279832No Hit
GANTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2890.15298128229016683No Hit
GANTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2870.15192258829507918No Hit
GANTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2760.14609977132209706No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2670.1413356483442026No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2500.13233674938595746No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2360.12492589142034387No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2230.11804438045227407No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.11380960447192343No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2070.10957482849157278No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1930.10216397052595919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)