FastQCFastQC Report
Wed 8 May 2024
EGAF00008118668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55430
Total Bases16.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15882.864874616633592No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14772.664622045823561No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12132.1883456611942993No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11602.0927295688255456No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10451.8852606891574961No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10401.876240303084972No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7891.423416922244272No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7681.3855313007396717No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7281.3133682121594805No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5130.9254916110409526No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4920.8876059895363522No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3930.7090023453003789No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3530.6368392567201876No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3240.584521017499549No Hit
GANTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2880.5195742377773769No Hit
GANTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2660.47988453905827166No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2560.4618437669132239No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2560.4618437669132239No Hit
GANTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2400.4329785314811474No Hit
GANTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2150.3878766011185279No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2050.36983582897348005No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1980.3572072884719466No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1980.3572072884719466No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1600.2886523543207649No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1550.27963196824824105No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1540.27782789103373623No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.27602381381923147No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1510.27241565939022194No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1490.2688075049612123No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1350.24355042395814544No Hit
GACTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1320.23813819231463107No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1230.22190149738408804No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1200.2164892657405737No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1180.21288111131156415No Hit
GACTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1130.20386072523904025No Hit
GACTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1050.189428107523002No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1040.1876240303084972No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.18221179866498285No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC910.16417102651993506No Hit
GACTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC900.16236694930543027No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG900.16236694930543027No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA800.14432617716038246No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT770.13891394551686811No Hit
GACTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC760.13710986830236332No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC760.13710986830236332No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG750.13530579108785856No Hit
GACTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC750.13530579108785856No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT750.13530579108785856No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.1335017138733538No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG690.12448132780082986No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT690.12448132780082986No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA680.12267725058632509No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC670.12087317337182031No Hit
GANTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC670.12087317337182031No Hit
GANTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC660.11906909615731553No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG660.11906909615731553No Hit
GACTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC660.11906909615731553No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA660.11906909615731553No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC650.11726501894281074No Hit
GACTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC650.11726501894281074No Hit
GANTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC600.10824463287028685No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC600.10824463287028685No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT590.10644055565578207No Hit
GACTACTGGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT590.10644055565578207No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT580.1046364784412773No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG580.1046364784412773No Hit
GACTACTCGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT570.1028324012267725No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT560.10102832401226772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)