FastQCFastQC Report
Wed 8 May 2024
EGAF00008118682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182771
Total Bases55 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG101905.575282730848985No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG91334.996963413232953No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG75694.141247790951519No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG72313.9563169211745843No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG60793.326019992230715No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG59053.230818893588151No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG58683.2105749818078357No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG53052.9025392430965526No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG51042.792565560181867No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG45972.515169255516466No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG43012.353217961273944No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG42542.3275027219854354No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG39782.1764940827593No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG34651.8958149815889829No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG33661.841648839257869No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30341.6600007659858511No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26951.4745227634580977No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG26131.4296578778909128No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24381.3339096464975297No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG23101.2638766543926552No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG19191.0499477488222968No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG19151.0477592178190194No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG15610.854074224028976No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG15030.822340524481455No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG14150.7741928424093537No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13870.7588731253864125No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12810.7008770537995634No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11600.6346739909504243No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10640.5821492468717685No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10570.5783193176160332No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9750.533454432048848No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8670.47436409496036025No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8080.4420832626620197No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7540.41253809411777576No Hit
GACTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7140.3906527840850026No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6850.3747859343112419No Hit
GACTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6690.36603181029813264No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5780.31624272997357344No Hit
GACTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5620.3074886059604642No Hit
GACTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5070.27739630466540094No Hit
GACTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4650.25441672913098906No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4290.23471995010149313No Hit
GACTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4140.22651295883920317No Hit
GACTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3960.21666456932445521No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3860.21119324181626187No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3810.20845757806216525No Hit
GACTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3650.19970345404905593No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3170.17344108200972802No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3090.1690640200031734No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3050.16687548899989604No Hit
GACTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2990.16359269249498007No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2940.1608570287408834No Hit
GANTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2800.15319717022941276No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2790.15265003747859343No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2640.14444304621630347No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2510.13733032045565213No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2490.1362360549540135No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2300.1258405326884462No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2270.12419913443598819No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2190.11982207242943355No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2160.11818067417697556No Hit
GANTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2110.1154450104228789No Hit
GANTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1980.10833228466222761No Hit
GACTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.10778515191140825No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1920.1050494881573116No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1830.10012529339993763No Hit
GANTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1830.10012529339993763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)