FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172978
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG45272.6170958156528576No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG33891.9592086854975777No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG31881.8430089375527523No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28881.6695764779336102No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG28781.6637953959463054No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23011.3302269652788217No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG22601.3065245291308722No Hit
CCTACGGGCGGCAGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG21571.2469793846616333No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20831.2041993779555782No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18971.09667125299171No Hit
CCTACGGGAGGCTGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG18871.0908901710044052No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT18341.06025043647169No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17681.0220952953554787No Hit
CCTACGGGAGGCAGCAGTGGGGAAGGCGGCTTTCTGGACGATGACTGACG16470.9521442033090913No Hit
CCTACGGGTGGCTGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG15580.9006925736220792No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12900.7457595763623119No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG12590.7278382222016673No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12580.7272601140029368No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC12330.7128074090346749No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11830.6839019990981512No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11400.6590433465527408No Hit
CCTACGGGCGGCTGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG11300.6532622645654361No Hit
CCTACGGGGGGCTGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG10750.62146631363526No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10620.6139509070517638No Hit
CCTACGGGTGGCAGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG10420.6023887430771543No Hit
CCTACGGGCGGCTGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG9570.5532495461850641No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9350.5405311658129935No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC9250.5347500838256888No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8960.517984946062505No Hit
CCTACGGGGGGCTGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG8620.49832926730566895No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC8570.4954387263120166No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC8310.48040791314502423No Hit
CCTACGGGTGGCAGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG8130.47000196556787566No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8080.46711142457422333No Hit
CCTACGGGGGGCAGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG7790.4503462868110396No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7790.4503462868110396No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7490.43300304084912533No Hit
CCTACGGGCGGCAGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG7080.4093006047011759No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6790.39253546693799213No Hit
CCTACGGGAGGCTGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG6630.3832857357583045No Hit
CCTACGGGGGGCAGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG6450.37287978818115597No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6430.37172357178369503No Hit
CCTACGGGCGGCAGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG6420.3711454635849646No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC6030.34859924383447605No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5750.33241221427002277No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC5590.3231624830903352No Hit
CCTACGGGAGGCAGCAGTGGGGAATGCGGCTTTCTGGACGATGACTGACG5390.3116003191157257No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC5280.30524112892969046No Hit
CCTACGGGAGGCTGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG5180.29946004694238576No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5050.29194464035888956No Hit
CCTACGGGAGGCAGCAGTGGCGAAGGCGGCTTTCTGGACGATGACTGACG4850.2803824763842801No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4660.269398420608401No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG4660.269398420608401No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4640.26824220421094014No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC4380.2532113910439478No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3930.22719652210107644No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3640.21043138433789266No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3510.2029159777543965No Hit
CCTACGGGCGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG3310.19135381377978702No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT3260.18846327278613464No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3210.1855727317924823No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3180.18383840719629085No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3140.18152597440136897No Hit
CCTACGGGGGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG3060.17690110881152515No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3040.1757448924140642No Hit
CCTACGGGTGGCAGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG3020.17458867601660327No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2860.1653389448369157No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2770.1601359710483414No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2760.15955786284961093No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2740.15840164645214996No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2730.15782353825341952No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2670.15435488906103664No Hit
CCTACGGGGGGCAGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG2410.13932407589404433No Hit
CCTACGGGTGGCTGCAGTGGGGAATGCGGCTTTATGGACGATGACTGACG2280.13180866931054816No Hit
CCTACGGGCGGCAGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG2280.13180866931054816No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2280.13180866931054816No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2210.12776191191943484No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2150.124293262727052No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2090.12082461353466914No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1990.1150435315473644No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1980.11446542334863394No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1870.10810623316259871No Hit
CCTACGGGGGGCTGCAGTGGGGAATGCGGCTTTATGGACGATGACTGACG1780.10290325937402445No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1770.10232515117529398No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1760.10174704297656349No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1760.10174704297656349No Hit
CCTACGGGTGGCTGCAGAATCATCTCGTATGCCGTCTTCTGCTTGAAAAA1760.10174704297656349Illumina PCR Primer Index 7 (96% over 28bp)
CCTACGGGAGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG1730.10001271838037207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)