FastQCFastQC Report
Wed 8 May 2024
EGAF00008118688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130294
Total Bases39.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG71435.482217139699449No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG68025.220501327766436No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG51993.990206763166378No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG51153.925737178995196No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG45213.4698451194989794No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG40073.0753526639753175No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG39513.0323729411945295No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG35022.687767663898568No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG29222.2426205350975486No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13031.0000460497029795No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12320.9455539011773375No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11120.8534544952185058No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10090.7744025051038421No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9400.7214453466775139No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9270.7114679110319738No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8180.6278109506193684No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7990.6132285446758868No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7920.6078560793282883No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7460.5725513070440695No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7430.5702488218950987No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7250.556433911001274No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7130.547223970405391No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7080.5433864951571062No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6800.5218966337667121No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6120.469706970390041No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6110.46893947534038405No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6080.4666369901914133No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5650.4336347030561653No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5390.41367983176508516No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5380.4129123367154282No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5070.38911999017606336No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5030.38605000997743566No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4950.3799100495801802No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4860.37300259413326786No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4760.36532764363669856No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4680.3591876832394431No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4590.35228022779253076No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4350.3338603466007644No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4340.3330928515511075No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4240.3254179010545382No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4130.31697545550831197No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3800.29164811886963327No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3760.28857813867100557No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3750.2878106436213486No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3550.27246074262821No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3410.2617158119330131No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3360.25787833668472837No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3300.25327336638678677No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3260.25020338618815907No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3150.24176094064193285No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2980.22871352479776505No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2750.21106113865565568No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2610.20031620796045865No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2590.1987812178611448No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2580.19801372281148788No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2430.18650129706663393No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2320.17805885152040768No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2120.1627089505272691No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2090.1604064653782983No Hit
GACTACTGGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG2020.1550340000306998No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2000.15349900993138593No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2000.15349900993138593No Hit
GACTACTCGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1920.1473590495341305No Hit
GACTACTGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1830.14045159408721813No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1830.14045159408721813No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1730.13277664359064884No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1730.13277664359064884No Hit
GACTACTCGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG1700.13047415844167803No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1690.1297066633920211No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1560.11972922774648104No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1510.11589175249819639No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1490.11435676239888252No Hit
GACTACCGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1450.11128678220025481No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1430.10975179210094095No Hit
GACTACTAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1400.10744930695197015No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1380.1059143168526563No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1340.10284433665402858No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1340.10284433665402858No Hit
GACTACCGGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG1330.10207684160437164No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1310.10054185150505779No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)