FastQCFastQC Report
Wed 8 May 2024
EGAF00008118718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87195
Total Bases26.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC18412.111359596307128No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC18242.091863065542749No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13381.5344916566316875No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12741.461092952577556No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12511.4347152933081027No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11211.285624175698148No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9261.061987499283216No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8771.0057916164917713No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8220.942714605195252No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6860.7867423590802225No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6330.7259590572853948No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4790.5493434256551408No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4770.5470497161534492No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4710.5401685876483743No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4160.477091576351855No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4000.45874190033832213No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3800.43580480532140603No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3650.41860198405871896No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3440.3945180342909571No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3280.3761683582774242No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3280.3761683582774242No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3240.3715809392740409No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3090.3543781180113538No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3010.3452032800045874No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2780.3188256207351339No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2640.30276965422329266No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2570.29474167096737197No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2530.29015425196398875No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2410.2763919949538391No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2300.26377659269453524No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2150.24657377143184814No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2070.2373989334250817No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2060.23625207867423592No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1980.22707724066746948No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1950.22363667641493207No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1900.21790240266070302No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1850.212168128906474No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1840.21102127415562819No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1840.21102127415562819No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1820.20872756465393658No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA1810.2075807099030908No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1790.20528700040139913No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1750.20069958139801594No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1710.1961121623946327No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1700.19496530764378692No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1690.1938184528929411No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG1660.19037788864040367No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.1892310338895579No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1620.18579046963702045No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1590.18234990538448306No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1580.18120305063363723No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1560.17890934113194565No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1560.17890934113194565No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1510.17317506737771662No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA1500.1720282126268708No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1500.1720282126268708No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG1490.17088135787602501No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1470.16858764837433338No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1420.16285337462010435No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1340.1536785366133379No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC1330.1525316818624921No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1330.1525316818624921No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG1300.1490911176099547No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC1290.1479442628591089No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1280.1467974081082631No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1250.14335684385572567No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG1250.14335684385572567No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1240.14220998910487986No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1230.14106313435403406No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1210.13876942485234245No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA1210.13876942485234245No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1190.13647571535065084No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG1180.13532886059980503No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1180.13532886059980503No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1180.13532886059980503No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1170.13418200584895923No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC1170.13418200584895923No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1150.13188829634726762No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1150.13188829634726762No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1140.13074144159642181No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1140.13074144159642181No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1120.1284477320947302No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG1100.1261540225930386No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC1090.1250071678421928No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.1250071678421928No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG1070.12271345834050118No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1060.12156660358965538No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC1050.12041974883880956No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC1040.11927289408796375No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC1030.11812603933711796No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1020.11697918458627216No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1010.11583232983542634No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA1010.11583232983542634No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1000.11468547508458053No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG990.11353862033373474No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT990.11353862033373474No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.11239176558288894No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG980.11239176558288894No Hit
GACTACACGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC970.11124491083204312No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC970.11124491083204312No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA970.11124491083204312No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG970.11124491083204312No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA960.11009805608119731No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT950.10895120133035151No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC940.10780434657950572No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA940.10780434657950572No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC930.1066574918286599No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG930.1066574918286599No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC920.10551063707781409No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA920.10551063707781409No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA910.10436378232696829No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG910.10436378232696829No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT910.10436378232696829No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA900.1032169275761225No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA890.10207007282527668No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC890.10207007282527668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)