FastQCFastQC Report
Wed 8 May 2024
EGAF00008118730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57992
Total Bases17.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9761.6829907573458407No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9051.5605600772520347No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7401.2760380742171336No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6501.1208442543799146No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6301.0863567388605324No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6211.0708373568768106No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6011.0363498413574286No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5750.9915160711822321No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5160.8897779004000551No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4920.8483928817767968No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4640.800110360049662No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4480.7725203476341564No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3930.677679679955856No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3830.660435922196165No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3750.6466409159884122No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3620.6242240309008139No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3200.5518002483101118No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3080.5311077389984825No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2950.5086908539108843No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2890.49834459925506963No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2490.42936956821630573No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2370.40867705890467654No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2320.400055180024831No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2000.34487515519381984No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1740.30004138501862326No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1670.2879707545868396No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1630.2810732514829632No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1510.26038074217133395No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1460.2517588632914885No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1290.22244447510001378No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1080.1862325838046627No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1080.1862325838046627No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1070.1845082080286936No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1000.17243757759690992No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC980.16898882604497173No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA960.16554007449303354No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC960.16554007449303354No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC880.15174506828528073No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA850.14657194095737344No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG850.14657194095737344No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA840.14484756518140435No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA840.14484756518140435No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG830.14312318940543522No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA830.14312318940543522No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA820.14139881362946613No Hit
GACTACTAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA800.13795006207752794No Hit
GACTACTCGGGTATCTAATCCCAAGCCTACACATCTGACGCTGCCGACGA790.13622568630155882No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA780.13450131052558975No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA770.13277693474962063No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG770.13277693474962063No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.13277693474962063No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA760.13105255897365153No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG760.13105255897365153No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA760.13105255897365153No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC750.12932818319768244No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG750.12932818319768244No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA750.12932818319768244No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA740.12760380742171334No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC740.12760380742171334No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC740.12760380742171334No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.12587943164574425No Hit
GACTACTGGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT730.12587943164574425No Hit
GACTACTGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA730.12587943164574425No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA720.12415505586977514No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA720.12415505586977514No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG720.12415505586977514No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA710.12243068009380603No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA710.12243068009380603No Hit
GACTACCCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA710.12243068009380603No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA700.12070630431783695No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA690.11898192854186784No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG680.11725755276589873No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA680.11725755276589873No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC670.11553317698992965No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA670.11553317698992965No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA670.11553317698992965No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG670.11553317698992965No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA670.11553317698992965No Hit
GACTACTCGGGTATCTAATCCACTATGTACACATCTGACGCTGCCGACGA650.11208442543799145No Hit
GACTACCAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA650.11208442543799145No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG650.11208442543799145No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG640.11036004966202234No Hit
GACTACTCGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT640.11036004966202234No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA630.10863567388605326No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA630.10863567388605326No Hit
GACTACTAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA620.10691129811008415No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG620.10691129811008415No Hit
GACTACTGGGGTATCTAATCCCAAGCCTACACATCTGACGCTGCCGACGA620.10691129811008415No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.10518692233411504No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG610.10518692233411504No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC610.10518692233411504No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC600.10346254655814593No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG590.10173817078217685No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA590.10173817078217685No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG590.10173817078217685No Hit
GACTACCCGGGTATCTAATCCCAAGCCTACACATCTGACGCTGCCGACGA580.10001379500620775No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG580.10001379500620775No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG580.10001379500620775No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA580.10001379500620775No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA580.10001379500620775No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG580.10001379500620775No Hit
GACTACTAGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA580.10001379500620775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)