FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61356
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11481.8710476563009324No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11291.8400808396896797No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9111.4847773648868896No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8021.3071256274854943No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7661.2484516591694372No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7011.1425125497098898No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6231.0153856183584327No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6090.9925679640132995No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5690.927374665884347No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3620.5899993480670187No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3340.544364039376752No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3230.5264358823912901No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3190.519916552578395No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2690.43842492991720455No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2630.42864593519786165No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2520.41071777821239974No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2460.4009387834930569No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2440.39767911858660926No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2320.37812112914792356No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2150.3504139774431188No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2010.32759632309798553No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1930.31455766347219505No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1870.3047786687528522No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1780.2901101766738379No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1720.2803311819544951No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1690.27544168459482365No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1650.2689223547819284No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1620.264032857422257No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1590.2591433600625856No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1490.2428450355303475No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1490.2428450355303475No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1460.23795553817067605No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1450.23632570571745226No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1410.22980637590455702No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1380.2249168785448856No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1370.22328704609166178No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1360.22165721363843793No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1310.2135080513723189No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1300.2118782189190951No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1270.2069887215594237No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.2069887215594237No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1260.20535888910619987No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA1250.20372905665297608No Hit
GACTACTCGGGTATCTAATCCTACAATGACACATCTGACGCTGCCGACGA1230.20046939174652845No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1230.20046939174652845No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1180.19232022948040942No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1180.19232022948040942No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.18417106721429038No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1130.18417106721429038No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1110.18091140230784275No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1110.18091140230784275No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1100.17928156985461896No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.17765173740139514No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1060.17276224004172372No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1050.1711324075884999No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1050.1711324075884999No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1010.16461307777560466No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG990.16135341286915705No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC970.15809374796270942No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA960.15646391550948563No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA960.15646391550948563No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA930.15157441814981418No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG920.1499445856965904No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG920.1499445856965904No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA910.14831475324336657No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA900.14668492079014278No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA900.14668492079014278No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC890.14505508833691896No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG890.14505508833691896No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG880.14342525588369515No Hit
GACTACTGGGGTATCTAATCCTACAATGACACATCTGACGCTGCCGACGA880.14342525588369515No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG870.14179542343047136No Hit
GACTACTAGGGTATCTAATCCTACAATGACACATCTGACGCTGCCGACGA870.14179542343047136No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC860.14016559097724754No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG860.14016559097724754No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA860.14016559097724754No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA840.13690592607079993No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC840.13690592607079993No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA830.13527609361757612No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC830.13527609361757612No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG810.1320164287111285No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG810.1320164287111285No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG800.1303865962579047No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC790.12875676380468087No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG780.12712693135145706No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA780.12712693135145706No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG780.12712693135145706No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA780.12712693135145706No Hit
GACTACTGGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA780.12712693135145706No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA770.12549709889823327No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG770.12549709889823327No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA770.12549709889823327No Hit
GACTACCGGGGTATCTAATCCTACAATGACACATCTGACGCTGCCGACGA760.12386726644500945No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC750.12223743399178565No Hit
GACTACCCGGGTATCTAATCCTACAATGACACATCTGACGCTGCCGACGA740.12060760153856183No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC740.12060760153856183No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA730.11897776908533803No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA720.11734793663211421No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA720.11734793663211421No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA720.11734793663211421No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC720.11734793663211421No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG720.11734793663211421No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG710.11571810417889042No Hit
GACTACCAGGGTATCTAATCCTACAATGACACATCTGACGCTGCCGACGA710.11571810417889042No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC700.11408827172566659No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA700.11408827172566659No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA700.11408827172566659No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC700.11408827172566659No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT700.11408827172566659No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA690.1124584392724428No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC690.1124584392724428No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG690.1124584392724428No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC690.1124584392724428No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG680.11082860681921897No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA680.11082860681921897No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA680.11082860681921897No Hit
GACTACTAGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA680.11082860681921897No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACGCTGCCGACGA680.11082860681921897No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA670.10919877436599518No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA660.10756894191277137No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA660.10756894191277137No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA650.10593910945954756No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC650.10593910945954756No Hit
GACTACTGGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA650.10593910945954756No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC640.10430927700632375No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG640.10430927700632375No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA640.10430927700632375No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA630.10267944455309994No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA630.10267944455309994No Hit
GACTACTGGGGTATCTAATCCGAAGGCCTTCTTCCTCCACGCGGCGTCGC630.10267944455309994No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG630.10267944455309994No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC620.10104961209987615No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA620.10104961209987615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)