FastQCFastQC Report
Wed 8 May 2024
EGAF00008118743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79558
Total Bases23.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT23212.9173684607456196No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16022.013625279670178No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15371.9319238794338722No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14431.813771085245984No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12581.5812363307272683No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10261.2896251791146083No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7520.9452223535031047No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6430.8082153900299153No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5330.6699514819377058No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4820.6058473063676815No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4280.5379722969405968No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4250.5342014630835366No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4050.5090625707031348No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3740.4700972875135122No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3630.4562708967042912No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3360.4223333919907489No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3260.409763945800548No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3200.4022222780864275No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3180.39970838884838733No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3010.3783403303250459No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2930.3682847733728852No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2910.365770884134845No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2790.350687548706604No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2700.3393750471354232No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2450.30795143165992106No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2350.2953819854697202No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2310.29035420699363984No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2010.2526458684230373No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1800.22625003142361547No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1790.2249930868045954No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1770.2224791975665552No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1670.20990975137635437No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1490.18728474823399283No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1450.18225696975791247No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1440.18100002513889238No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1380.17345835742477186No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1370.17220141280575177No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1280.160888911234571No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1240.15586113275849064No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1210.15209029890143042No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1210.15209029890143042No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1190.14957640966339025No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1180.14831946504437016No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1180.14831946504437016No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGGT1150.1445486311873099No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1140.1432916865682898No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1060.1332361296161291No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1040.13072224037808894No Hit
CCTACGGGCGGCTGCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACG1040.13072224037808894No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTTGCTGGACGATGACTGACG1030.12946529575906884No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1020.12820835114004878No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG970.12192362804494833No Hit
CCTACGGGAGGCAGCAGTAGGGAAGGCGGCTTTCTGGACGACAACTGACG930.11689584956886799No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT910.11438196033082783No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT900.11312501571180773No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT900.11312501571180773No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT880.11061112647376757No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT870.10935418185474748No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT860.10809723723572738No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT840.10558334799768722No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT830.10432640337866712No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG830.10432640337866712No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT830.10432640337866712No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGGT820.10306945875964706No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT810.10181251414062696No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT800.10055556952160688No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT800.10055556952160688No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC800.10055556952160688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)