FastQCFastQC Report
Wed 8 May 2024
EGAF00008118745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59978
Total Bases18 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13752.292507252659308No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10321.720630897995932No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9561.5939177698489446No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8671.4455300276768148No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8331.3888425756110574No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6461.0770615892493915No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT6221.0370469172029746No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6131.022041415185568No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5370.8953282870385808No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4770.7952916069225383No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4660.7769515489012638No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4380.7302677648471106No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4110.6852512587948915No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3910.6519056987562106No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3870.6452365867484744No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3830.6385674747407383No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3790.631898362733002No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3750.6252292507252659No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3680.6135583047117277No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT3530.588549134682717No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3070.5118543465937511No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2770.46183600653572976No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2740.45683417252992764No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2690.4484977825202574No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT2670.4451632265163893No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2660.4434959485144553No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2640.4401613925105873No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2380.3968121644603021No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2310.3851412184467638No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2270.3784721064390277No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2220.3701357164293574No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2210.3684684384274234No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2190.3651338824235553No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2000.3334556003868085No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1970.3284537663810064No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1930.3217846543732702No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1930.3217846543732702No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1920.32011737637133614No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1900.3167828203674681No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1860.3101137083597319No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1600.26676448030944677No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1570.26176264630364465No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1510.2517589782920404No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1400.23341892027076597No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT1390.2317516422688319No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1370.22841708626496382No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1310.21841341825335958No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1250.2084097502417553No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1230.20507519423788723No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT1160.19340424822434893No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1160.19340424822434893No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT1100.1834005802127447No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT1080.18006602420887657No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT1080.18006602420887657No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1060.1767314682050085No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1060.1767314682050085No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT1030.17172963419920637No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1020.17006235619727234No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT990.1650605221914702No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT960.16005868818566807No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT940.1567241321818No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT930.15505685417986595No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG930.15505685417986595No Hit
CCTACGGGTGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT930.15505685417986595No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT920.15338957617793192No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT910.15172229817599786No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT910.15172229817599786No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT910.15172229817599786No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT880.14672046417019574No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT870.14505318616826168No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT840.14005135216245956No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT830.1383840741605255No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT830.1383840741605255No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT820.1367167961585915No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT810.13504951815665744No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT800.13338224015472339No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGACTGGAT780.13004768415085532No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT780.13004768415085532No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT770.12838040614892127No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT770.12838040614892127No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT760.12671312814698724No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT760.12671312814698724No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT760.12671312814698724No Hit
CCTACGGGTGGCAGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT740.12337857214311915No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT740.12337857214311915No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTCTTCACGGGAT730.1217112941411851No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT730.1217112941411851No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT730.1217112941411851No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT720.12004401613925106No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT720.12004401613925106No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT720.12004401613925106No Hit
CCTACGGGTGGCAGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT700.11670946013538298No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT690.11504218213344894No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT690.11504218213344894No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG690.11504218213344894No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT680.11337490413151488No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT680.11337490413151488No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG680.11337490413151488No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT670.11170762612958084No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT670.11170762612958084No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG670.11170762612958084No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG660.11004034812764682No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT660.11004034812764682No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT660.11004034812764682No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG650.10837307012571276No Hit
CCTACGGGGGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT650.10837307012571276No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT650.10837307012571276No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT650.10837307012571276No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGACTGGAT640.10670579212377872No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT640.10670579212377872No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGACGTGTCTTCACGGGAT640.10670579212377872No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT640.10670579212377872No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT630.10503851412184467No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT630.10503851412184467No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT620.10337123611991064No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG620.10337123611991064No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT620.10337123611991064No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT620.10337123611991064No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGTTGTGTACATAGAGGAT620.10337123611991064No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGCATAGCGGAT610.1017039581179766No Hit
CCTACGGGCGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT610.1017039581179766No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT600.10003668011604255No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT600.10003668011604255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)