FastQCFastQC Report
Wed 8 May 2024
EGAF00008118746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59978
Total Bases18 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10471.7456400680249424No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9651.6089232718663509No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7651.2754676714795425No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7551.258794891460202No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7451.2421221114408616No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6581.0970689252726No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6301.0503851412184466No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5520.9203374570675915No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5520.9203374570675915No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5500.9170029010637234No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5360.8936610090366467No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5280.8803227850211746No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5070.8453099469805596No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4690.781953382907066No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4490.7486078228683851No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4390.7319350428490446No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4170.6952549268064957No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4120.6869185367968255No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3930.6552402547600787No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3490.5818800226749808No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3400.5668745206575745No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3340.5568708526459701No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3230.5385307946246958No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3160.5268598486111574No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3090.5151889025976192No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2730.4551668945279936No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2540.42348861249124675No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2500.4168195004835106No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2450.40848311047384045No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2100.3501283804061489No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2100.3501283804061489No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2040.34012471239454467No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2000.3334556003868085No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1990.33178832238487443No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.2884390943345893No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1690.2817699823268532No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1610.26843175831138083No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1590.26509720230751277No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1510.2517589782920404No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1470.24508986628430426No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1400.23341892027076597No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1390.2317516422688319No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1360.22674980826302976No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1350.22508253026109573No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1320.22008069625529364No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1210.20174063823401914No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1170.19507152622628296No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1140.19006969222048084No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1120.18673513621661275No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.1850678582146787No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.1850678582146787No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1110.1850678582146787No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1100.1834005802127447No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1100.1834005802127447No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA1090.18173330221081063No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1070.17839874620694254No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1060.1767314682050085No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1040.17339691220114042No Hit
GACTACAAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA970.16172596618760213No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA970.16172596618760213No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA950.15839141018373404No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA930.15505685417986595No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA930.15505685417986595No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC860.14338590816632765No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC850.14171863016439362No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG840.14005135216245956No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA830.1383840741605255No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA800.13338224015472339No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA790.13171496215278936No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA790.13171496215278936No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA780.13004768415085532No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC780.13004768415085532No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA780.13004768415085532No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC770.12838040614892127No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA770.12838040614892127No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.12838040614892127No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA760.12671312814698724No Hit
GACTACTCGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA760.12671312814698724No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA750.12504585014505318No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA750.12504585014505318No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC740.12337857214311915No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA740.12337857214311915No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA740.12337857214311915No Hit
GACTACTGGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA740.12337857214311915No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC740.12337857214311915No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.12337857214311915No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA730.1217112941411851No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACGTCTGACGCTGCCGACGA720.12004401613925106No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA720.12004401613925106No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA720.12004401613925106No Hit
GACTACTGGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT720.12004401613925106No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC710.11837673813731701No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG710.11837673813731701No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC710.11837673813731701No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA680.11337490413151488No Hit
GACTACTGGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA680.11337490413151488No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA680.11337490413151488No Hit
GACTACTCGGGTATCTAATCCAGTCCAAACACATCTGACGCTGCCGACGA680.11337490413151488No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACGTCTGACGCTGCCGACGA670.11170762612958084No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA660.11004034812764682No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA660.11004034812764682No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA650.10837307012571276No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA630.10503851412184467No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA630.10503851412184467No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA630.10503851412184467No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA620.10337123611991064No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA620.10337123611991064No Hit
GACTACTCGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT620.10337123611991064No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA610.1017039581179766No Hit
GACTACTAGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA610.1017039581179766No Hit
GACTACTAGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT610.1017039581179766No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA610.1017039581179766No Hit
GACTACTGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA600.10003668011604255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)