FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89236
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT20952.347707203370837No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13981.5666323008651217No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13071.4646555201936438No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12071.3525931238513604No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10251.1486395625084047No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8400.9413241292751805No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT7830.8774485633600788No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6550.7340086960419562No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6130.6869424895781971No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5250.5883275807969878No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4640.5199695190281949No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4540.5087632793939666No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4420.49531579183289254No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4280.4796270563449729No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4250.4762651844547044No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3770.4224752342104084No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3680.4123896185396029No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3400.3810121475637635No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3360.3765296517100722No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3100.3473934286610785No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2930.32834282128289033No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2930.32834282128289033No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2850.31937782957550764No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2780.31153346183154784No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2770.31041283786812496No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2690.30144784616074227No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2680.30032722219731944No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2610.29248285445335964No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2590.290241606526514No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2560.28687973463624544No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2540.28463848670939984No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2530.28351786274597696No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2510.2812766148191313No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2480.2779147429288628No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2260.25326101573356047No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2190.2454166479896006No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2150.24093415213590927No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2100.23533103231879512No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1980.22188354475772112No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1970.22076292079429827No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1900.21291855305033844No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1840.20619480926980144No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1790.20059168945268727No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1790.20059168945268727No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1760.19722981756241875No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1640.18378233000134475No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1630.18266170603792192No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1610.18042045811107624No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1560.17481733829396207No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1560.17481733829396207No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1530.17145546640369358No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1490.16697297055000224No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1450.16249047469631092No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1450.16249047469631092No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1430.16024922676946524No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1420.1591286028060424No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1400.15688735487919672No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1380.15464610695235106No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1380.15464610695235106No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1340.15016361109865972No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1330.14904298713523692No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1300.1456811152449684No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1280.14343986731812272No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGCACAATGGGGGAGACCCTGAT1250.14007799542785423No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1230.13783674750100858No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1230.13783674750100858No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1190.13335425164731723No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1180.13223362768389438No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1170.13111300372047158No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1150.1288717557936259No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG1120.12550988390335738No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1120.12550988390335738No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1090.12214801201308888No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1080.12102738804966606No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1060.11878614012282038No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1050.11766551615939756No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1050.11766551615939756No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1040.11654489219597472No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT1040.11654489219597472No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGATAACTGACG1020.11430364426912906No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1020.11430364426912906No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTACACAATGGGGGAAACCCTGAT1020.11430364426912906No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1010.11318302030570622No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1000.11206239634228338No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1000.11206239634228338No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT950.10645927652516922No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT950.10645927652516922No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC930.10421802859832355No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC930.10421802859832355No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAGTGGGGGAAACCCTGAT930.10421802859832355No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG910.10197678067147788No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT900.10085615670805505No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT900.10085615670805505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)