FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89236
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC21522.411582769285938No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19532.1885786005647945No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15331.7179165359272042No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15171.6999865525124387No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14481.6226634990362632No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12891.4444842888520328No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10461.1721726657402842No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10451.1710520417768613No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9331.045542157873504No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4390.4919539199426241No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3670.41126899457618005No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3580.40118337890537453No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3310.370926531892958No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3230.3619615401855753No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3080.3451521807342328No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2950.330584069209736No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2870.3216190775023533No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2810.31489533372181633No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2780.31153346183154784No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2680.30032722219731944No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2570.28800035859966827No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2510.2812766148191313No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2390.2678291272580573No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2380.26670850329463447No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2280.2555022636604061No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2220.24877851987986913No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2210.24765789591644627No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2200.24653727195302344No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2150.24093415213590927No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2140.23981352817248644No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2120.23757228024564075No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2050.22972791250168095No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2050.22972791250168095No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2020.22636604061141244No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1970.22076292079429827No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1960.21964229683087544No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1960.21964229683087544No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1950.21852167286745258No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1840.20619480926980144No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1790.20059168945268727No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1780.19947106548926444No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1780.19947106548926444No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1760.19722981756241875No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1760.19722981756241875No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1750.1961091935989959No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1720.19274732170872744No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1710.19162669774530458No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1710.19162669774530458No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1700.19050607378188175No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1680.1882648258550361No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1680.1882648258550361No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1660.1860235779281904No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1600.1792998341476534No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1530.17145546640369358No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1510.16921421847684792No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1510.16921421847684792No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1500.16809359451342507No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1490.16697297055000224No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1460.16361109865973372No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1450.16249047469631092No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1430.16024922676946524No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1420.1591286028060424No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1400.15688735487919672No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1400.15688735487919672No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1350.15128423506208258No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1330.14904298713523692No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1320.14792236317181406No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1300.1456811152449684No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1290.14456049128154558No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1290.14456049128154558No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1280.14343986731812272No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1270.14231924335469992No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1260.14119861939127706No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1250.14007799542785423No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1240.1389573714644314No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1240.1389573714644314No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1220.13671612353758572No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1180.13223362768389438No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1140.12775113183020306No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCCTCACGCGGCATTGC1130.12663050786678023No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1130.12663050786678023No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1120.12550988390335738No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1110.12438925993993456No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1110.12438925993993456No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1100.12326863597651172No Hit
GACTACTGGGGTATCTAATCCGAGGACCTTCTTCCTCCACGCGGCGTCGC1100.12326863597651172No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1100.12326863597651172No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1090.12214801201308888No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1080.12102738804966606No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1060.11878614012282038No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1060.11878614012282038No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1060.11878614012282038No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1050.11766551615939756No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1040.11654489219597472No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC1040.11654489219597472No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1030.11542426823255188No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1020.11430364426912906No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1020.11430364426912906No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1010.11318302030570622No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTTGC980.10982114841543772No Hit
GACTACCAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA980.10982114841543772No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.10982114841543772No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT980.10982114841543772No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA940.10533865256174638No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCCTCACGCGGCATTGC930.10421802859832355No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA930.10421802859832355No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA920.10309740463490072No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA920.10309740463490072No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC920.10309740463490072No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA900.10085615670805505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)