FastQCFastQC Report
Wed 8 May 2024
EGAF00008118760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62366
Total Bases18.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13022.087675977295321No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12892.0668312862777793No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9871.582593079562582No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9211.476766186704294No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8801.4110252381105088No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8291.3292499118109227No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6881.1031651861591252No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6811.0919411217650643No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6251.0021486066125773No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3510.5628066574736235No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3440.5515825930795626No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2760.4425488246801142No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2720.43613507359779363No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2650.42491100920373276No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2620.42010069589199245No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2310.3703941250040086No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2100.33672193182182597No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2070.3319116185100856No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2050.3287047429689254No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1950.31267036526312414No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1890.3030497386396434No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1850.2966359875573229No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1840.29503254978674276No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1840.29503254978674276No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1810.2902222364750024No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1680.2693775454574608No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.2645672321457204No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1630.2613603566045602No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1570.25173972998107946No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1530.24532597889875893No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1520.24372254112817882No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1500.24051566558701856No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1490.23891222781643845No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1430.2292916011929577No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1390.22287785011063718No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1350.2164640990283167No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1330.21325722348715645No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1310.21005034794599622No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1230.1972228457813552No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.19561940801077507No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.19561940801077507No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1220.19561940801077507No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1200.19241253246961484No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1200.19241253246961484No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1170.18760221915787448No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1120.17958503030497386No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1060.16996440368149313No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1060.16996440368149313No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1030.16515409036975276No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1020.16355065259917262No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.15713690151685214No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA960.1539300259756919No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA940.15072315043453163No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG940.15072315043453163No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.1394990860404708No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT860.13789564826989065No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT850.13629221049931053No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG850.13629221049931053No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA840.1346887727287304No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.13148189718757017No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT820.13148189718757017No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA800.12827502164640991No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA760.12186127056408941No Hit
GACTACTGGGGTATCTAATCCGCTGTGTACACATCTGACGCTGCCGACGA760.12186127056408941No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG760.12186127056408941No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA750.12025783279350928No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG740.11865439502292917No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT720.11544751948176891No Hit
GACTACTCGGGTATCTAATCCGCTGTGTACACATCTGACGCTGCCGACGA720.11544751948176891No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT720.11544751948176891No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA710.11384408171118879No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG710.11384408171118879No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG710.11384408171118879No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT690.11063720617002855No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT660.10582689285828815No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT650.10422345508770804No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA650.10422345508770804No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT640.10262001731712793No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA640.10262001731712793No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG640.10262001731712793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)