FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008118764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26554
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4091.5402575883106122No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3461.303005196957144No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2701.016795962943436No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2630.990434586126384No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2610.9829027641786549No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2230.8397981471718009No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2050.7720117496422384No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1850.6966935301649468No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.62514122166152No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1650.6213753106876554No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1560.5874821119228741No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1540.579950289975145No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1510.5686525570535512No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1500.5648866460796866No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1420.53475935828877No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1370.5159298034194472No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1270.47827069368080144No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1230.46320704978534305No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1220.45944113881147847No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1220.45944113881147847No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1190.44814340588988477No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1110.41801611809896816No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.3803570083603224No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG990.3728251864125932No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.36529336446486405No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC960.36152745349099946No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG960.36152745349099946No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC940.35399563154327035No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.34646380959554113No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA910.3426978986216766No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA890.33516607667394743No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG830.31257061083076No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC830.31257061083076No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA800.30127287790916624No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG790.29750696693530165No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG790.29750696693530165No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA780.29374105596143707No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC780.29374105596143707No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG750.2824433230398433No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG750.2824433230398433No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG690.2598478571966559No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG670.25231603524892676No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA670.25231603524892676No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA650.24478421330119757No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG640.24101830232733296No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA610.22972056940573923No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA610.22972056940573923No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA610.22972056940573923No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.22972056940573923No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.22972056940573923No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA610.22972056940573923No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA600.22595465843187468No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA590.2221887474580101No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA590.2221887474580101No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA570.21465692551028093No Hit
GACTACTGGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA570.21465692551028093No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA560.21089101453641637No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA550.2071251035625518No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA520.19582737064095807No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA510.19206145966709345No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA510.19206145966709345No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG510.19206145966709345No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA490.18452963771936431No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA480.18076372674549973No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG480.18076372674549973No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG470.17699781577163518No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA460.17323190479777056No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA460.17323190479777056No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG450.169465993823906No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG450.169465993823906No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA440.16570008285004142No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA440.16570008285004142No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA440.16570008285004142No Hit
GACTACTCGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA430.16193417187617684No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA430.16193417187617684No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA430.16193417187617684No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA420.15816826090231229No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG420.15816826090231229No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG420.15816826090231229No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA410.1544023499284477No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA400.15063643895458312No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC400.15063643895458312No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA400.15063643895458312No Hit
GACTACCGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA390.14687052798071853No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA380.14310461700685395No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG380.14310461700685395No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG380.14310461700685395No Hit
GACTACAGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA370.13933870603298937No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG370.13933870603298937No Hit
GACTACTGGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA370.13933870603298937No Hit
GACTACTCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA370.13933870603298937No Hit
GACTACAAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA370.13933870603298937No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA370.13933870603298937No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG370.13933870603298937No Hit
GACTACCAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA360.1355727950591248No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA350.13180688408526023No Hit
GACTACACGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA350.13180688408526023No Hit
GACTACCCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA350.13180688408526023No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG340.12804097311139565No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG340.12804097311139565No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA340.12804097311139565No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC330.12427506213753108No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG330.12427506213753108No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA330.12427506213753108No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA330.12427506213753108No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA320.12050915116366648No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA320.12050915116366648No Hit
GACTACAAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA310.11674324018980191No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC310.11674324018980191No Hit
GACTACCAGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA300.11297732921593734No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG300.11297732921593734No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA300.11297732921593734No Hit
GACTACCGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA300.11297732921593734No Hit
GACTACTAGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA290.10921141824207276No Hit
GACTACTGGGGTATCTAATCCGCTATGCACACATCTGACGCTGCCGACGA290.10921141824207276No Hit
GACTACTGGGGTATCTAATCCTGTGAAGACACATCTGACGCTGCCGACGA290.10921141824207276No Hit
GACTACTCGGGTATCTAATCCCAAGCCTACACATCTGACGCTGCCGACGA290.10921141824207276No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA290.10921141824207276No Hit
GACTACCCGGGTATCTAATCCGCTATGCACACATCTGACGCTGCCGACGA290.10921141824207276No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA290.10921141824207276No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC280.10544550726820819No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT280.10544550726820819No Hit
GACTACAGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA280.10544550726820819No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG280.10544550726820819No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC280.10544550726820819No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC280.10544550726820819No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA280.10544550726820819No Hit
GACTACCAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA270.10167959629434359No Hit
GACTACCCGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA270.10167959629434359No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG270.10167959629434359No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCG270.10167959629434359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)