FastQCFastQC Report
Wed 8 May 2024
EGAF00008118782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45263
Total Bases13.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7901.7453549256567173No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7521.661401144422597No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5721.2637253385767626No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5211.1510505269204427No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5111.128957426595674No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4881.0781432958487065No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3950.8726774628283587No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3890.8594216026334975No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3540.7820957514968075No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3450.7622119612045158No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3100.6848861100678257No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3050.6738395599054415No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2550.5633740582815986No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2440.5390716479243532No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2380.5258157877294921No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2380.5258157877294921No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2130.4705830369175707No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1940.42860614630051036No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1780.3932571857808806No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1780.3932571857808806No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1770.3910478757484038No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1740.3844199456509732No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1700.37558270552106576No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1670.3689547754236352No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1610.35569891522877406No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1580.3490709851313435No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1560.34465236506638974No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1530.33802443496895923No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.30488478448180634No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1350.29825685438437577No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1300.28721030422199145No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1300.28721030422199145No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1270.2805823741245609No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1220.2695358239621766No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1130.2496520336698849No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.24523341360493117No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1080.2386054835075006No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1080.2386054835075006No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1010.2231403132801626No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA990.2187216932152089No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG970.21430307315025518No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG930.20546583302034774No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA870.1922099728254866No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA860.19000066279300973No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG850.18779135276053288No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG840.18558204272805603No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC790.17453549256567175No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG790.17453549256567175No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.17232618253319487No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC770.17011687250071802No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA740.16348894240328746No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA690.15244239224090317No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG690.15244239224090317No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA670.14802377217594945No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG650.14360515211099573No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG640.14139584207851888No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA640.14139584207851888No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA630.13918653204604203No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG630.13918653204604203No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG620.13697722201356516No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC610.1347679119810883No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA600.13255860194861144No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA590.1303492919161346No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA590.1303492919161346No Hit
GACTACTAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA590.1303492919161346No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG580.12813998188365774No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA570.12593067185118087No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCA560.12372136181870402No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCA560.12372136181870402No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA550.12151205178622716No Hit
GACTACTCGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA550.12151205178622716No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA540.1193027417537503No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC530.11709343172127344No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA530.11709343172127344No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA520.1148841216887966No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG520.1148841216887966No Hit
GACTACTGGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA520.1148841216887966No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC500.11046550162384287No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA500.11046550162384287No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG500.11046550162384287No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA500.11046550162384287No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA500.11046550162384287No Hit
GACTACCCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA490.10825619159136601No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA480.10604688155888915No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC480.10604688155888915No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG470.1038375715264123No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA470.1038375715264123No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC470.1038375715264123No Hit
GACTACCAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA470.1038375715264123No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA470.1038375715264123No Hit
GACTACTAGGGTATCTAATCCTACGACAACACATCTGACGCTGCCGACGA470.1038375715264123No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG460.10162826149393545No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA460.10162826149393545No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA460.10162826149393545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)