FastQCFastQC Report
Wed 8 May 2024
EGAF00008118796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170000
Total Bases51.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA48252.8382352941176467No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA43652.567647058823529No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA37452.202941176470588No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG37322.195294117647059No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA35072.0629411764705883No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG32071.886470588235294No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA29911.7594117647058822No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA27761.6329411764705883No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA27541.6199999999999999No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27491.6170588235294117No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG26701.5705882352941176No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG23711.3947058823529412No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA23201.3647058823529412No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20541.208235294117647No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA20201.188235294117647No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20191.1876470588235293No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18871.11No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG17581.0341176470588236No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG15820.9305882352941176No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14830.8723529411764707No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG13410.7888235294117647No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13400.788235294117647No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13230.778235294117647No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12430.7311764705882353No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA11610.6829411764705883No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG11450.6735294117647059No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10580.6223529411764706No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG10380.6105882352941177No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA10270.6041176470588235No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG10070.5923529411764706No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9400.5529411764705883No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA9310.5476470588235295No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9080.5341176470588236No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8400.49411764705882355No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA8330.49No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8160.48No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8040.4729411764705882No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7990.47000000000000003No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7920.46588235294117647No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7750.45588235294117646No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7380.43411764705882355No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7350.4323529411764706No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7070.4158823529411764No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA6830.40176470588235297No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA6750.39705882352941174No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA6560.38588235294117645No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6450.3794117647058824No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6090.35823529411764704No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6030.3547058823529412No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5960.35058823529411764No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5790.34058823529411764No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5750.338235294117647No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5690.3347058823529412No Hit
GANTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5660.33294117647058824No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5650.33235294117647063No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5350.31470588235294117No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5220.3070588235294118No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5120.3011764705882353No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5100.3No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4800.2823529411764706No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4640.27294117647058824No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4440.2611764705882353No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4140.24352941176470588No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4030.2370588235294118No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3960.23294117647058823No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3940.23176470588235293No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3750.22058823529411764No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3650.21470588235294116No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3620.21294117647058822No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3460.20352941176470588No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3450.20294117647058824No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3400.2No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3360.1976470588235294No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3320.19529411764705884No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3210.18882352941176472No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3160.18588235294117647No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2980.17529411764705882No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2940.17294117647058824No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2890.16999999999999998No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2880.16941176470588237No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2780.16352941176470587No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2730.16058823529411764No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2710.15941176470588236No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2680.15764705882352942No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2500.14705882352941177No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2460.14470588235294118No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2450.14411764705882352No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2330.13705882352941176No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2330.13705882352941176No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2310.13588235294117648No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2240.13176470588235295No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2220.13058823529411764No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2180.12823529411764706No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2180.12823529411764706No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2080.12235294117647058No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.12176470588235294No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2070.12176470588235294No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2040.12No Hit
GACTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2040.12No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2040.12No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2000.1176470588235294No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1940.11411764705882353No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1900.11176470588235295No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1880.11058823529411764No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1830.10764705882352942No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1830.10764705882352942No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1800.10588235294117646No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1790.10529411764705883No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1790.10529411764705883No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1780.10470588235294118No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1770.10411764705882352No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1750.10294117647058824No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1740.10235294117647059No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.10235294117647059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)