FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93597
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12631.3494022244302704No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12441.3291024284966397No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12311.3152130944367875No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11211.1976879600841908No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9981.0662734916717416No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9711.0374264132397406No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9691.035289592615148No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9350.9989636419970727No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9320.9957584110601835No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9180.9808006666880349No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8980.9594324604421082No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8760.9359274335715888No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8630.9220380995117365No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8030.8579334807739564No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7860.8397705054649188No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7760.8290864023419555No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7690.8216075301558811No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7290.7788711176640277No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7160.7649817836041753No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7070.7553660907935084No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7010.7489556289197303No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6730.7190401401754329No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6680.7136980886139512No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6520.69660352361721No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6380.6816457792450613No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5610.5993781851982435No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5610.5993781851982435No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5600.5983097748859473No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5480.5854888511383912No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5450.5822836202015022No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5330.5694626964539462No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5230.5587785933309828No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5000.5342051561481671No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4850.5181790014637222No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4730.505358077716166No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4530.4839898714702394No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3350.357917454619272No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3070.3280019658749746No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2870.30663375962904793No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2770.2959496565060846No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2740.2927444255691956No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2670.28526555338312126No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2600.2777866811970469No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2580.2756498605724543No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2540.27137621932326894No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2420.2585552955757129No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2390.2553500646388239No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2370.25321324401423123No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2270.2425291408912679No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2180.23291344808060088No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2160.2307766274560082No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2130.2275713965191192No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2130.2275713965191192No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2100.2243661655822302No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2070.22116093464534117No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2010.21475047277156317No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2000.21368206245926685No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1990.21261365214697053No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1980.21154524183467416No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1940.20727160058548888No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1900.20299795933630352No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1890.2019295490240072No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1880.20086113871171082No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1880.20086113871171082No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1870.1997927283994145No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.19872431808711818No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1830.19551908715022917No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1830.19551908715022917No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.1773561118411915No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1640.17521929121659882No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1630.1741508809043025No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1570.16774041903052447No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1540.1645351880936355No Hit
GANTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.16346677778133914No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1510.16132995715674647No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1500.16026154684445015No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1480.1581247262198575No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1470.15705631590756114No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1460.1559879055952648No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1430.1527826746583758No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1430.1527826746583758No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1340.1431669818477088No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.1399617509108198No Hit
GANTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1280.1367565199739308No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.13568810966163447No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1260.13461969934933812No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1250.13355128903704178No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1250.13355128903704178No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1210.12927764778785644No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1190.12714082716326378No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1150.12286718591407846No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1130.12073036528948577No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.11966195497718944No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1110.11859354466489311No Hit
GANTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1080.1153883137280041No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1070.11431990341570777No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.11431990341570777No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1070.11431990341570777No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1040.11111467247881877No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1020.10897785185422611No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1010.10790944154192976No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG980.10470421060504076No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG980.10470421060504076No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA970.10363580029274444No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG970.10363580029274444No Hit
GACTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG940.10043056935585541No Hit
GANTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC940.10043056935585541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)