FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71996
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10431.4486915939774432No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT6970.9681093394077449No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6770.9403300183343519No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6180.8583810211678428No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5820.8083782432357353No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5320.7389299405522529No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5090.706983721317851No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4990.6930940607811545No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4360.6055891993999666No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4130.5736429801655648No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3940.5472526251458414No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3890.5403077948774931No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3720.5166953719651092No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3650.5069726095894216No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT3620.5028057114284127No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3540.4916939829990555No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3140.43613534085226957No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT2980.4139118839935552No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT2830.3930773931885105No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2730.379187732651814No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2700.37502083449080503No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2690.3736318684371354No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2580.35835324184676925No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2570.35696427579309964No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2570.35696427579309964No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2500.3472415134174121No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT2370.32918495471970666No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2360.327795988666037No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2330.3236290905050281No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2260.31390632812934055No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2220.3083504639146619No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2140.2972387354853048No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2070.2875159731096172No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT2040.2833490749486083No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1860.2583476859825546No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1770.24584699149952774No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1760.2444580254458581No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1710.23751319517750988No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1700.23612422912384023No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1620.225012500694483No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1580.21945663647980446No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT1490.2069559419967776No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1490.2069559419967776No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT1460.20278904383576865No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1460.20278904383576865No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1420.19723317962109005No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1400.19445524751375076No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1360.1888993832990722No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT1320.18334351908439356No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1320.18334351908439356No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1320.18334351908439356No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCGTATGTGTAGACTTGGGAT1310.18195455303072391No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1310.18195455303072391No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1290.17917662092338463No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1240.1722317906550364No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1200.16667592644035778No Hit
CCTACGGGTGGCAGCAGTAGTCGGCAGCGTCAGATGTGTACATAGAGGAT1200.16667592644035778No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1180.1638979943330185No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1150.15973109617200956No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1120.1555641980110006No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1120.1555641980110006No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1110.15417523195733096No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1100.15278626590366132No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1100.15278626590366132No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT1100.15278626590366132No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1090.15139729984999165No Hit
CCTACGGGCGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1080.15000833379632203No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1010.14028557142063447No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT1000.13889660536696483No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT970.1347297072059559No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT960.13334074115228625No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT940.13056280904494694No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT930.1291738429912773No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT920.12778487693760762No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT920.12778487693760762No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT920.12778487693760762No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT910.126395910883938No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT890.1236179787765987No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACTCTGAT890.1236179787765987No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG880.12222901272292905No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT880.12222901272292905No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT870.12084004666925939No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT860.11945108061558976No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG850.11806211456192012No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT850.11806211456192012No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT840.11667314850825046No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT840.11667314850825046No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATGGAGGAT830.1152841824545808No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT820.11389521640091116No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT820.11389521640091116No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT820.11389521640091116No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT820.11389521640091116No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT810.1125062503472415No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT800.11111728429357187No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT800.11111728429357187No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGCATAGCGGAT780.10833935218623257No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT760.10556142007889327No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT740.10278348797155398No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT730.10139452191788433No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTTGCTGGACGATGACTGACG730.10139452191788433No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGAGGAT730.10139452191788433No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTGTCGTAGGAT720.10000555586421468No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACACAGCGGAT720.10000555586421468No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGCATAGCGGAT720.10000555586421468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)