FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008118832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58767
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG30105.121922167202682No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG28464.842853982677353No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25494.337468307043069No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25374.317048683785118No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG24144.1077475453911205No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22963.9069545833546035No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22463.8218728197798084No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20643.5121752003675533No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19753.3607296612044175No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7831.3323804175812957No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7521.2796297241649224No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7291.2404921129205166No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6721.1434989024452498No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6701.1400956319022582No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6431.0941514795718685No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6411.0907482090288767No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6381.085643303214389No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6321.0754334915854136No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6201.0550138683274626No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5710.9716337400241631No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5650.9614239283951878No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5500.9358993993227491No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5240.8916568822638556No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5000.8508176357479538No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4890.8320996477614988No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4770.8116800245035478No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4710.8014702128745724No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4540.7725424132591421No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4070.6925655554988344No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3970.6755492027838753No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3800.6466214031684449No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3770.6415164973539571No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3700.6296050504534858No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3610.6142903330100227No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3470.59046743920908No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3290.5598380043221536No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3250.5530314632361699No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3220.5479265574216822No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3160.5377167457927068No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3110.5292085694352273No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3100.5275069341637313No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2910.4951758640053091No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2840.48326441710483775No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2630.4475300764034237No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2470.42030391205948914No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2230.3794646655435874No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2010.34202868957067745No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2000.34032705429918153No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1890.3216090663127265No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1870.31820579576973473No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1850.3148025252267429No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1820.30969761941225515No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1790.30459271359776746No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1490.2535436554528902No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1490.2535436554528902No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1450.24673711436690662No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1430.2433338438239148No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1430.2433338438239148No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1420.24163220855241888No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1390.23652730273793116No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1370.23312403219493935No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1340.2280191263804516No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1200.20419623257950892No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG740.12592101009069714No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG730.12421937481920126No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG680.11571119846172172No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG650.110606292647234No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG590.10039648101825854No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)