FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122967
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT44693.634308391682321No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT37733.0683028780079207No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT34312.790179479047224No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT30392.4713947644489984No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT30212.456756690819488No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT27532.238812039002334No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT26552.1591158603527774No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT25972.1119487342132444No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT22101.7972301511787716No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT21371.7378646303479794No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19311.570340009921361No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16291.3247456634706873No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15721.2783917636439044No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15401.2523685216358862No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14641.1905633218668423No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12591.0238519277529743No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT12140.9872567436791985No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT9030.7343433604137696No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT8370.6806704237722316No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT7730.6286239397561948No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT7310.5944684346206706No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT6900.5611261557978969No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA6380.5188383875348671No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT6040.49118869290134753No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT5350.4350760773215578No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5170.4204380036920474No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5140.4179983247537957No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT4850.394414761684029No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT4840.3936015353712785No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4740.3854692722437727No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4540.36920474598876124No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT4480.3643253881122578No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4440.36107248286125543No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4200.34155505135524167No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT4090.3326095619149853No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT3930.3195979409109761No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3730.3033334146559646No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3580.291135019964706No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3480.2830027568372002No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3470.2821895305244496No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3330.2708043621459416No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT3320.269991135833191No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3190.25941919376743355No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3170.2577927411419324No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2950.23990176226141974No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT2900.2358356306976669No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2820.22932982019566225No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2750.22363723600640822No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT2670.21713142550440365No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2550.2073727097513967No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2520.20493303081314498No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2480.20168012556214268No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2420.19680076768563926No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2380.19354786243463692No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2360.19192140980913577No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2150.17484365724137368No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2150.17484365724137368No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2120.17240397830312199No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2100.17077752567762083No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2090.16996429936487026No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2080.16915107305211968No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2020.16427171517561623No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1910.1553262257353599No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1890.15369977310985874No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1850.15044686785885644No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1850.15044686785885644No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1740.1415013784186001No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1720.13987492579309896No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1650.13418234160384493No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1630.13255588897834378No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1630.13255588897834378No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1610.13092943635284263No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1560.12686330478908975No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1540.1252368521635886No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1470.11954426797433457No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1450.11791781534883344No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1450.11791781534883344No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1430.11629136272333228No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT1410.11466491009783113No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1390.11303845747232996No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1370.11141200484682884No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1320.10734587328307595No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1300.10571942065757481No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1280.10409296803207364No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGGGCAACCCTGAT1270.10327974171932307No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1270.10327974171932307No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1250.10165328909382192No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT1230.10002683646832078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)