FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008118850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122967
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG42773.478168939634211No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG40213.2699830035700637No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG32132.6128961428675987No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG31592.5689819219790673No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG25632.084299039579725No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG24291.9753267136711476No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23761.9322257190953671No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21061.7126546146527115No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19011.5459432205388437No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC18451.5004025470248115No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17731.44185025250677No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14281.1612871746078217No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12811.0417429066334871No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11350.9230118649719031No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10950.89048281246188No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG9930.8075337285613213No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9550.7766311286767994No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG9550.7766311286767994No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG9400.7644327339855408No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG9310.7571136971707857No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9110.7408491709157742No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8680.7058804394674995No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG7560.614799092439435No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG7430.6042271503736775No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG7370.5993477924971741No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG7340.5969081135589224No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG6190.5033870875926061No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5880.47817707189733827No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5520.44890092463831754No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5470.44483479307456475No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5360.4358893036343084No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5170.4204380036920474No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4760.38709572486927385No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG4650.37815023542901754No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4570.37164442492701294No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4440.36107248286125543No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4420.3594460302357543No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3920.31878471459822555No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG3800.30902599884521864No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3750.30495986728146574No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3660.2976408304667106No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3280.2667382305821887No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3260.26511177795668756No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3170.2577927411419324No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3100.25210015695267834No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3100.25210015695267834No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3070.24966047801442665No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3000.24396789382517262No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2960.24071498857417029No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2920.23746208332316798No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2900.2358356306976669No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2870.2333959517594151No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2740.22282400969365768No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2600.21143884131514962No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2590.210625615002399No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2480.20168012556214268No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2400.1951743150601381No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2360.19192140980913577No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2350.1911081834963852No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2330.18948173087088407No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2270.18460237299438062No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2230.1813494677433783No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2180.17728333617962544No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2170.17647010986687486No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2160.1756568835541243No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2140.1740304309286231No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2110.1715907519903714No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2070.1683378467393691No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2050.16671139411386796No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2030.1650849414883668No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCAG1950.1585791309863622No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1930.15695267836086105No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1910.1553262257353599No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG1900.15451299942260932No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCAG1900.15451299942260932No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1880.15288654679710817No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG1860.15126009417160702No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1840.14963364154610587No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1830.1488204152333553No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1830.1488204152333553No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1770.14394105735685184No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1760.14312783104410126No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1730.14068815210584953No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.14068815210584953No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCAG1650.13418234160384493No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1650.13418234160384493No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1610.13092943635284263No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCAG1610.13092943635284263No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1580.1284897574145909No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1560.12686330478908975No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1540.1252368521635886No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1470.11954426797433457No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1460.11873104166158399No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG1450.11791781534883344No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.11629136272333228No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1420.1154781364105817No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG1420.1154781364105817No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1410.11466491009783113No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1400.11385168378508055No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1380.11222523115957939No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCAG1330.10815909959582652No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTAAG1300.10571942065757481No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1280.10409296803207364No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1270.10327974171932307No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1240.10084006278107134No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1240.10084006278107134No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1230.10002683646832078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)