FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90420
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC38984.310993143109931No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC25672.838973678389737No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC24162.6719752267197525No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC23602.6100420261004205No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC22952.538155275381553No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC20912.312541473125415No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC17571.9431541694315417No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC13771.5228931652289315No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13691.5140455651404556No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12471.3791196637911967No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10791.1933200619332005No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8640.9555408095554082No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8590.9500110595001107No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8240.9113028091130281No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7990.8836540588365406No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7770.8593231585932316No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7330.8106613581066137No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7220.7984959079849591No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7060.780800707808007No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6940.7675293076752931No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6870.7597876575978766No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6800.7520460075204601No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6620.7321389073213891No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6430.7111258571112585No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6220.6879009068790091No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6220.6879009068790091No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5700.630391506303915No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5500.6082725060827251No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5240.579517805795178No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4820.533067905330679No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4620.5109489051094891No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3970.4390621543906215No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3250.3594337535943375No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT3230.35722185357221853No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT2010.2222959522229595No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1860.20570670205706704No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1760.19464720194647203No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1750.19354125193541252No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1720.19022340190223402No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1680.18579960185799602No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1630.18026985180269853No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1550.1714222517142225No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1530.16921035169210352No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1480.163680601636806No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1470.16257465162574652No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1410.1559389515593895No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1410.1559389515593895No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1360.150409201504092No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1300.143773501437735No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1290.1426675514266755No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1290.1426675514266755No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1280.14156160141561602No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1210.13381995133819952No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT1120.123866401238664No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1110.12276045122760451No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1050.1161247511612475No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1010.11170095111700952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)