FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008118879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31273
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8482.711604259265181No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6802.1743996418635883No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6342.0273079013845807No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5501.7587055926837847No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5211.665973843251367No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4141.323825664311067No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3391.0840021743996417No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2910.9305151408563297No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2350.751446935055799No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2150.6874940044127522No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2010.6427269529626195No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2000.6395293064304671No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1870.5979599015124868No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1820.5819716688517251No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1730.5531928500623541No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1620.5180187382086784No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1440.46046110062993634No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1420.45406580756563164No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1400.44767051450132705No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGACGAGGGGAT1390.4444728679691747No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1360.43487992837271766No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1240.3965081699868897No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1240.3965081699868897No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1220.390112876922585No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1220.390112876922585No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1160.37092699772967097No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1130.36133405813321395No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1110.35493876506890926No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1100.35174111853675694No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1070.3421481789402999No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1020.32615994627953826No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1010.32296229974738594No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1010.32296229974738594No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT990.31656700668308124No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC950.3037764205544719No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC940.3005787740223196No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT930.29738112749016726No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT900.28778818789371025No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT900.28778818789371025No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT880.28139289482940555No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT870.27819524829725323No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT850.27179995523294853No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT830.2654046621686439No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT820.26220701563649157No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT810.2590093691043392No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC790.2526140760400345No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT770.24621878297572986No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT760.24302113644357753No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT750.2398234899114252No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT740.23662584337927287No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT720.23023055031496817No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT720.23023055031496817No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT710.22703290378281582No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT700.22383525725066353No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT700.22383525725066353No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT690.22063761071851118No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGCACATAGCGGAT680.21743996418635883No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACAGGAT660.2110446711220542No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT660.2110446711220542No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT630.20145173152559714No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT600.19185879192914015No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT600.19185879192914015No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT600.19185879192914015No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT580.18546349886483549No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT580.18546349886483549No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACAGGAT540.17267291273622615No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT540.17267291273622615No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTAGGAT540.17267291273622615No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT530.1694752662040738No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT530.1694752662040738No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT520.16627761967192145No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCATGGGAT510.16307997313976913No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT510.16307997313976913No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCATGGGAT490.15668468007546446No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT490.15668468007546446No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT480.1534870335433121No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT470.1502893870111598No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT470.1502893870111598No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT460.14709174047900744No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACAGGAT460.14709174047900744No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT450.14389409394685512No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGGCGC440.14069644741470277No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGGCGC430.13749880088255045No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTAGGAT430.13749880088255045No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT430.13749880088255045No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT430.13749880088255045No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT420.1343011543503981No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC420.1343011543503981No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT420.1343011543503981No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT410.13110350781824578No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT410.13110350781824578No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT400.12790586128609344No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGACAGGAT400.12790586128609344No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT400.12790586128609344No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGCACATAGCGGAT400.12790586128609344No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATAGGAT400.12790586128609344No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT400.12790586128609344No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT400.12790586128609344No Hit
CCTACGGGGGGCAGCAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAAT390.1247082147539411No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGCACATAGCGGAT390.1247082147539411No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACAGGAT390.1247082147539411No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT390.1247082147539411No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTAGGAT380.12151056822178877No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATAGGAT380.12151056822178877No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT380.12151056822178877No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT370.11831292168963643No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGCACATAGCGGAT370.11831292168963643No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC370.11831292168963643No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGTGAGGAT370.11831292168963643No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCATGGGAT360.11511527515748408No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGACAGGAT350.11191762862533176No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATAGGAT350.11191762862533176No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT350.11191762862533176No Hit
ACTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT340.10871998209317942No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT340.10871998209317942No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGGCGC340.10871998209317942No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT340.10871998209317942No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAGACTTGGGAT340.10871998209317942No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGAGGAT330.1055223355610271No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT330.1055223355610271No Hit
CCTACGGGTGGCTGCAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAAT330.1055223355610271No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTTGGTGAGGAT330.1055223355610271No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCGGATGTGTACATAGAGGAT330.1055223355610271No Hit
CCTACGGGTGGCAGCAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAAT330.1055223355610271No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGCACATAGCGGAT330.1055223355610271No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACCTAGGAT330.1055223355610271No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCGGATGTGTACATAGCGGAT330.1055223355610271No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT330.1055223355610271No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT320.10232468902887475No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAACAGC320.10232468902887475No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC320.10232468902887475No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT320.10232468902887475No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCATGGGAT320.10232468902887475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)