FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008118885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56690
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT28124.960310460398659No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT21253.7484565179043923No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT19453.430940201093667No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17853.1487034750396896No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT16602.9282060328100195No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11712.0656200388075496No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9481.6722526018698183No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8581.5134944434644557No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7701.3582642441347679No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6001.0583877227024168No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5921.0442758863997177No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5540.977244663961898No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4700.8290703827835597No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4540.800846710178162No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3880.6844240606808961No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3500.6173928382430763No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3110.5485976362674193No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2960.5221379431998588No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2720.47980243429176217No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2650.46745457752690067No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2630.463926618451226No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2320.4092432527782678No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2040.3598518257188217No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2040.3598518257188217No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1830.3228082554242371No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1800.317516316810725No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1590.2804727465161404No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1510.26636091021344155No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1460.2575410125242547No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1430.25224907391074264No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1400.24695713529723057No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1360.23990121714588108No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1310.2310813194566943No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1270.22402540130534487No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1250.22049744222967013No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1180.20814958546480863No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1180.20814958546480863No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1160.2046216263891339No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1060.18698183101076027No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1050.1852178514729229No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1050.1852178514729229No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1010.17816193332157346No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1000.17639795378373613No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT980.1728699947080614No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT910.16052213794319986No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT900.1587581584053625No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT900.1587581584053625No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT890.15699417886752515No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG890.15699417886752515No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC890.15699417886752515No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT880.1552301993296878No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT870.1534662197918504No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT860.15170224025401305No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC860.15170224025401305No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT820.1446463221026636No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT820.1446463221026636No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT790.13935438348915152No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT770.1358264244134768No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC760.13406244487563942No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT740.1305344857999647No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT740.1305344857999647No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC720.12700652672429No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT710.12524254718645264No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG710.12524254718645264No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT710.12524254718645264No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT700.12347856764861528No Hit
CCTACGGGTAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT700.12347856764861528No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT700.12347856764861528No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT700.12347856764861528No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC700.12347856764861528No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT700.12347856764861528No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT690.12171458811077791No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT680.11995060857294054No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT680.11995060857294054No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT670.1181866290351032No Hit
CCTACGGGTGGCAGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT660.11642264949726583No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG660.11642264949726583No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT650.11465866995942846No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC640.11289469042159112No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT640.11289469042159112No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC630.11113071088375374No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT630.11113071088375374No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT630.11113071088375374No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC630.11113071088375374No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT620.10936673134591637No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT620.10936673134591637No Hit
CCTACGGGTGGCTGCAGTGGTGAAGGCGGATTGCTGGACAGTAACTGACG620.10936673134591637No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT610.10760275180807903No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC610.10760275180807903No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT600.10583877227024166No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGACTGGAT590.10407479273240432No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT590.10407479273240432No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG590.10407479273240432No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT580.10231081319456695No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT580.10231081319456695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)