FastQCFastQC Report
Wed 8 May 2024
EGAF00008118889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96760
Total Bases29.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT21402.211657709797437No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14841.5336916081025216No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12681.3104588673005373No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12501.291856138900372No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12071.2474162877221993No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9240.954940057875155No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7290.753410500206697No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC6480.6696982224059529No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT6340.6552294336502686No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5910.6107895824720959No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5630.5818520049607275No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4760.49193881769326164No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4500.46506821000413395No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4480.4630012401818933No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4360.4505994212484498No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4260.4402645721372468No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4170.4309632079371641No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4060.4195948739148409No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3930.406159570070277No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3920.40512608515915666No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3760.38859032658123194No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3630.375155022736668No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3590.37102108309218684No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT3460.357585779247623No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3230.3338156262918562No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3210.33174865646961554No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2750.28420835055808186No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2680.2769739561802398No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2580.26663910706903676No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2360.24390243902439024No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2170.2242662257131046No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2040.21083092186854072No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2020.20876395204630013No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1910.19739561802397687No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1850.19119470855725507No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1830.18912773873501446No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1820.18809425382389416No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1660.17155849524596942No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1560.16122364613476642No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1550.16019016122364613No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1530.15812319140140554No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1520.15708970649028522No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1520.15708970649028522No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1480.15295576684580403No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1470.15192228193468377No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1460.15088879702356348No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1430.14778834229020257No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1350.13952046300124019No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1350.13952046300124019No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1270.1312525837122778No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG1230.1271186440677966No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1190.12298470442331541No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1190.12298470442331541No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1180.12195121951219512No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1170.12091773460107483No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1130.11678379495659365No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1120.11575031004547333No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1120.11575031004547333No Hit
CCTACGGGTGGCTGCAGAGAACTGACCCAGGAAAGATGCAGTCATGATTG1110.11471682513435305No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1110.11471682513435305No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1110.11471682513435305No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1100.11368334022323273No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1100.11368334022323273No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1090.11264985531211245No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1070.11058288548987186No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1070.11058288548987186No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1070.11058288548987186No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACAGGAT1060.10954940057875155No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1060.10954940057875155No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1060.10954940057875155No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1060.10954940057875155No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1050.10851591566763126No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1050.10851591566763126No Hit
CCTACGGGTGGCTGCAGAGGAGAAAGGGGTGGAGGGAGGCTGGCTCTTCT1030.10644894584539066No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1020.10541546093427036No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1010.10438197602315007No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1010.10438197602315007No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1000.10334849111202976No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1000.10334849111202976No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT990.10231500620090947No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT990.10231500620090947No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT970.10024803637866887No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAGACCCTGAT970.10024803637866887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)