FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008118898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86630
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC17492.0189310862287893No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15691.8111508715225673No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12861.4844742006233407No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12811.4787025279926123No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12291.418677132633037No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12261.4152141290546No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11581.336719381276694No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10221.179729885720882No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10201.1774212166685905No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9921.1450998499365115No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8701.004271037746739No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8691.0031167032205932No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8590.9915733579591366No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7830.9038439339720652No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7710.8899919196583169No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7270.8392012005079071No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6550.7560891146254184No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6530.7537804455731271No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6490.7491631074685444No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5740.6625880180076187No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5410.6244949786448113No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4770.550617568971488No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4600.5309938820270114No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4510.5206048712917004No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA4130.47674015929816455No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3930.45365346877525103No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3420.3947824079418215No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3250.37515872099734504No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3190.36823271384047096No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA3190.36823271384047096No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2900.3347570125822463No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2750.3174419946900612No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2550.2943553041671476No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2520.29089230058871063No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2490.2874292970102736No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2460.28396629343183655No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2430.2805032898533995No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2290.26434260648736No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2160.24933625764746625No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2120.24471891954288352No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2070.23894724691215516No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1950.22509523259840702No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1870.2158605563892416No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1790.20662588018007616No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1790.20662588018007616No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1780.2054715456539305No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1740.2008542075493478No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1720.19854553849705647No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.19392820039247374No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1670.19277386586632805No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1610.185847858709454No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1550.17892185155257992No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1540.17776751702643426No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1540.17776751702643426No Hit
GACTACAGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1510.17430451344799722No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1490.17199584439570587No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1490.17199584439570587No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1480.1708415098695602No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1460.16853284081726885No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1450.16737850629112316No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1440.1662241717649775No Hit
GACTACTAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1440.1662241717649775No Hit
GACTACACGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1430.1650698372388318No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1400.1616068336603948No Hit
GACTACAAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1350.1558351610296664No Hit
GACTACCCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1340.15468082650352072No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1310.1512178229250837No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1300.15006348839893802No Hit
GACTACCAGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1290.14890915387279233No Hit
GACTACTCGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1280.14775481934664667No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1260.14544615029435531No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1260.14544615029435531No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1240.14313748124206394No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1240.14313748124206394No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1210.13967447766362692No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1180.13621147408518988No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA1160.13390280503289853No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1140.13159413598060718No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.13043980145446152No Hit
GACTACCGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA1120.12928546692831583No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1110.12813113240217014No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA1110.12813113240217014No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA1090.1258224633498788No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA1090.1258224633498788No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1080.12466812882373313No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1070.12351379429758744No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1060.12235945977144176No Hit
GACTACTGGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA1060.12235945977144176No Hit
GACTACCAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1060.12235945977144176No Hit
GACTACCCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1050.12120512524529609No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA1040.12005079071915042No Hit
GACTACTGGGGTATCTAATCCGGTTATAACACATCTGACGCCGCCGACGA1040.12005079071915042No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1030.11889645619300472No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.11889645619300472No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1020.11774212166685905No Hit
GACTACTGGGGTATCTAATCCCGATATCATACATCTGACGCTGCCGACGT1010.11658778714071338No Hit
GACTACCGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1000.11543345261456771No Hit
GACTACTAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA990.11427911808842203No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA980.11312478356227634No Hit
GACTACTCGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA970.11197044903613067No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA970.11197044903613067No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA950.10966177998383933No Hit
GACTACTAGGGTATCTAATCCAATTAACACACATCTGACGCTGCCGACGA930.10735311093154797No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA920.10619877640540229No Hit
GACTACTCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC920.10619877640540229No Hit
GACTACTGGGGTATCTAATCCTGATATCATACATCTGACGCTGCCGACGA910.10504444187925661No Hit
GACTACTGGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC900.10389010735311092No Hit
GACTACAGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA900.10389010735311092No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG890.10273577282696525No Hit
GACTACACGGGTATCTAATCCGCTATGTACACGTCTGACGCTGCCGACGA890.10273577282696525No Hit
GACTACTGGGGTATCTAATCCTAAGTCTACACATCTGACGCTGCCGACGA880.10158143830081957No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCCGACGCTGCCGACGA880.10158143830081957No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC870.1004271037746739No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG870.1004271037746739No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)