FastQCFastQC Report
Wed 8 May 2024
EGAF00008118908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112228
Total Bases33.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC27452.4459136757315467No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC26932.399579427593827No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC21231.891684784545746No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19461.7339701322308159No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC18751.6707060626581602No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC17271.5388316641123427No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13921.2403321809174181No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13631.2144919271483052No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12291.0950921338703354No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA8680.773425526606551No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA8190.7297644081690844No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA7160.6379869551270628No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6330.5640303667533948No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5990.5337348968171935No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5300.47225291371137335No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4750.4232455358734006No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4310.38403963360302246No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4290.38225754713618704No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3710.33057703959796125No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3650.32523078019745516No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC3280.29226218056100084No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA3200.2851338346936593No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA2990.266421926791888No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2900.25840253769112875No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2770.24681897565669886No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA2710.24147271625619277No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2650.23612645685568667No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2530.2254339380546744No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2520.22454289482125672No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2450.21830559218733292No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA2440.21741454895391524No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2300.20493994368606766No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2250.20048472751897922No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2200.19602951135189078No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2190.1951384681184731No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2180.19424742488505542No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2180.19424742488505542No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA2170.19335638165163774No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA2170.19335638165163774No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2170.19335638165163774No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2060.1835549060840432No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2060.1835549060840432No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1990.1773176034501194No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1980.17642656021670172No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1960.17464447374986636No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1950.17375343051644865No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1900.16929821434936024No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1880.16751612788252485No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1820.16216986848201875No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1750.15593256584809495No Hit
GACTACTAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1750.15593256584809495No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1750.15593256584809495No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1740.15504152261467727No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1690.15058630644758886No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1680.14969526321417115No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1640.14613109028050042No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1640.14613109028050042No Hit
GACTACCCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1600.14256691734682966No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1590.14167587411341198No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1580.1407848308799943No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1560.1390027444131589No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1550.13811170117974125No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1540.13722065794632357No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1520.13543857147948818No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1470.13098335531239977No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1460.13009231207898206No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCCTCCTCCACGCGGCGTCGC1450.12920126884556438No Hit
GACTACAGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1410.12563709591189365No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1390.12385500944505827No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1370.1220729229782229No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.1220729229782229No Hit
GACTACCAGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1360.12118187974480522No Hit
GACTACACGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1330.11850875004455216No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.11761770681113447No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1320.11761770681113447No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.11672666357771679No Hit
GACTACCGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA1310.11672666357771679No Hit
GACTACTCGGGTATCTAATCCAAAGACCTTCTTCCTCCACGCGGCGTCGC1310.11672666357771679No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCCTCCTCCACGCGGCGTCGC1290.11494457711088142No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1280.11405353387746373No Hit
GACTACTGGGGTATCTAATCCAAAGACCTTCTTCCTCCACGCGGCGTCGC1270.11316249064404606No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1250.11138040417721068No Hit
GACTACTCGGGTATCTAATCCAACAATGACACATCTGACGCTGCCGACGA1250.11138040417721068No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1160.10336101507645151No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1160.10336101507645151No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1140.10157892860961613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)