FastQCFastQC Report
Wed 8 May 2024
EGAF00008118926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78045
Total Bases23.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC16612.128259337561663No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14951.9155615350118522No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12031.5414184124543533No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11531.4773528092766992No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11361.455570504196297No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9971.277468127362419No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8431.0801460695752452No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8181.048113267986418No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7170.9187007495675572No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6340.8123518482926516No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5590.7162534435261708No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4520.5791530527259914No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3930.5035556409763599No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3840.4920238324043821No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3490.4471779101800243No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2710.34723556922288423No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2660.34082900890511886No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2660.34082900890511886No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2240.2870139022358895No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1810.23191748350310717No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1780.22807354731244794No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1720.22038567493112945No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1610.2062912422320456No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1600.20500993016849253No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1580.2024473060413864No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1550.19860336985072716No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1520.19475943366006793No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1500.19219680953296175No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1470.18835287334230252No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1410.18066500096098403No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1380.1768210647703248No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1380.1768210647703248No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1360.17425844064321866No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1340.1716958165161125No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1320.16913319238900634No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1320.16913319238900634No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1320.16913319238900634No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1320.16913319238900634No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1280.16400794413479403No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1280.16400794413479403No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1200.1537574476263694No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1180.15119482349926325No Hit
GACTACTAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1180.15119482349926325No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1160.14863219937215708No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1150.14735088730860402No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1130.14478826318149784No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1130.14478826318149784No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1120.14350695111794476No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1110.14222563905439167No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1110.14222563905439167No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA1090.13966301492728553No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1080.13838170286373247No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1070.13710039080017938No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1070.13710039080017938No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1060.13581907873662633No Hit
GACTACCCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1060.13581907873662633No Hit
GACTACCGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1030.13197514254596707No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.12941251841886092No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1010.12941251841886092No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1000.12813120635530784No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA980.1255685822282017No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCCTCCTCCACGCGGCGTCGC980.1255685822282017No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA970.1242872701646486No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA960.12300595810109552No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA940.12044333397398937No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC930.11916202191043629No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT920.1178807098468832No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.1178807098468832No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA920.1178807098468832No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA910.11659939778333013No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA900.11531808571977706No Hit
GACTACCCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA900.11531808571977706No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG900.11531808571977706No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG890.11403677365622397No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC880.1127554615926709No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG880.1127554615926709No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC870.11147414952911781No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG860.11019283746556473No Hit
GACTACCAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA860.11019283746556473No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA860.11019283746556473No Hit
GACTACTAGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA850.10891152540201167No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA840.10763021333845858No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC830.1063489012749055No Hit
GACTACACGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA830.1063489012749055No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT830.1063489012749055No Hit
GACTACTGGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA830.1063489012749055No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA830.1063489012749055No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT830.1063489012749055No Hit
GACTACTCGGGTATCTAATCCCATTCGAACACATCTGACGCTGCCGACGA810.10378627714779935No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG810.10378627714779935No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT810.10378627714779935No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA800.10250496508424627No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACACTGCCGACGA800.10250496508424627No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG800.10250496508424627No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA790.1012236530206932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)