FastQCFastQC Report
Wed 8 May 2024
EGAF00008118933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98148
Total Bases29.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT41754.25378000570567No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT31323.191099156376085No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT27942.8467212780698534No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT25472.595060520846069No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT24502.4962301829889557No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT16681.6994742633573787No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15351.563964624852264No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14311.4580022007580389No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11781.2002282267595876No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11191.1401149284753638No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9881.006643028895138No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9300.9475486000733587No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8060.8212087867302441No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7160.7295105351102417No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5610.5715857684313486No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5510.5613970738069038No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC4540.4625667359497901No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4480.4564535191751233No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4290.43709499938867835No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4230.43098178261401154No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3850.3922647430411216No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3640.3708684843297877No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3600.3667930064800098No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3420.3484533561560093No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3260.33215144475689773No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3140.31992501120756406No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3120.3178872722826751No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2970.3026042303460081No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2960.30158536088356364No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2740.27917023270978525No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2720.2771324937848963No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2580.26286832131067367No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2580.26286832131067367No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2570.2618494518482292No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2520.25675510453600686No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2510.25573623507356236No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2440.24860414883645107No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2200.22415128173778376No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2140.21803806496311692No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2100.21396258711333904No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2050.20886823980111666No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2030.20683050087622773No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1860.18950972001467173No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1860.18950972001467173No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1810.18441537270244937No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1780.18135876431511594No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1760.179321025390227No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1620.1650568529160044No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1600.16301911399111546No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1560.15894363614133755No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1530.15588702775400415No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1510.1538492888291152No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1410.1436605942046705No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1390.14162285527978155No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1390.14162285527978155No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1380.1406039858173371No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1370.13958511635489262No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1370.13958511635489262No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1340.1365285079675592No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1330.13550963850511472No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG1250.12735868280555895No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1190.12124546603089212No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1160.1181888576435587No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1160.1181888576435587No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1150.11716998818111424No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1120.11411337979378082No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG1100.11207564086889188No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1090.1110567714064474No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1060.10800016301911398No Hit
CCTACGGGGGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG1060.10800016301911398No Hit
CCTACGGGTAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1050.10698129355666952No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1030.10494355463178057No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT990.10086807678200269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)