FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008118952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008118952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45194
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11412.52467141655972No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11192.4759923883701376No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10732.374208965791919No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10502.3233172545028102No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8261.8276762402088773No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7691.7015533035358676No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7661.694915254237288No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7611.6838518387396557No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7571.6750011063415498No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6861.5179006062751692No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6561.4515201132893747No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6111.3519493738106827No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6031.334247909014471No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5561.230251803336726No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5321.1771474089480904No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4971.0997035004646634No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4611.02004690888171No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4330.9580917820949683No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4110.9094127539053858No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3880.8585210426162764No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3680.8142673806257469No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3260.7213346904456344No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3230.7146966411470549No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3000.6638049298579457No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2990.6615922467584192No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2960.6549541974598398No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2890.6394654157631544No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2830.6261893171659955No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2770.6129132185688365No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2680.5929990706730982No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2470.546532725583042No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2380.5266185776873036No Hit
GANTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2140.47351418329866796No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2100.464663450900562No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2090.4624507678010355No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.4580254016019826No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2060.45581271850245614No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1870.4137717396114528No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1660.36730539452139666No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1660.36730539452139666No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1610.3562419790237642No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1590.3518166128247112No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.3385405142275523No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.3385405142275523No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1520.33632783112802583No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1430.31641368323228747No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1420.314201000132761No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1300.28764880293844314No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1210.26773465504270477No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1120.2478205071469664No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1110.24560782404743992No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG950.21020489445501617No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG930.20577952825596318No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC910.2013541620569102No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG880.19471611275833076No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG820.18144001416117184No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG780.17258928176306587No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG780.17258928176306587No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG780.17258928176306587No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG750.16595123246448643No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.16152586626543347No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG680.15046245076780101No Hit
GANTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC680.15046245076780101No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG670.14824976766827455No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG660.14603708456874806No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG650.14382440146922157No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG650.14382440146922157No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG610.13497366907111563No Hit
GANTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC590.13054830287206268No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG570.1261229366730097No Hit
GANTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC550.12169757047395671No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG540.11948488737443022No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG540.11948488737443022No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG520.11505952117537727No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG500.1106341549763243No Hit
GANTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC490.1084214718767978No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG490.1084214718767978No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG480.10620878877727132No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG480.10620878877727132No Hit
GACTANACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG470.10399610567774482No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG470.10399610567774482No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG470.10399610567774482No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG460.10178342257821836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)